EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.97 | cloning of cardiac muscle enzyme, expression in Escherichia coli, DNA and amino acid sequence determination | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.97 | m-calpain | enzyme is inactivated by cleavage, protease is specific for PEST regions, i.e. regions rich in proline, glutamic acid, serine and threonine, calpain-inhibitor calpastatin protects | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.97 | 0.0058 | - |
myristoyl-CoA | recombinant enzyme | Bos taurus | |
2.3.1.97 | 0.016 | - |
p60src-derived peptide | recombinant enzyme | Bos taurus | |
2.3.1.97 | 0.05 | - |
M2 gene segment of reovirus type 3-derived peptide | recombinant enzyme | Bos taurus | |
2.3.1.97 | 0.1 | - |
cAMP-dependent protein kinase-derived peptide | recombinant enzyme | Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.97 | cytosol | major part | Bos taurus | 5829 | - |
2.3.1.97 | mitochondrion | occasionally | Bos taurus | 5739 | - |
2.3.1.97 | myofibril | occasionally | Bos taurus | 30016 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.97 | additional information | - |
amino acid sequence, alignment | Bos taurus |
2.3.1.97 | 50000 | - |
1 * 50000, recombinant enzyme | Bos taurus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.97 | myristoyl-CoA + glycylpeptide | Bos taurus | enzyme is possibly regulated by calpains in vivo | N-myristoylglycylpeptide + CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.97 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.97 | cardiac muscle enzyme, recombinant from Escherichia coli | Bos taurus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.97 | tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein] | PEST regions for recognition by clapains, putative regulatory enzymes | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.1.97 | cardiac muscle | cardiac, low activity | Bos taurus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.97 | myristoyl-CoA + cAMP-dependent protein kinase-derived peptide | - |
Bos taurus | N-myristoylated cAMP-dependent protein kinase-derived peptide + CoA | - |
? | |
2.3.1.97 | myristoyl-CoA + Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Arg | pp60src-derived peptide | Bos taurus | N-myristoyl-Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Arg + CoA | - |
? | |
2.3.1.97 | myristoyl-CoA + glycylpeptide | substrate specificity | Bos taurus | N-myristoylglycylpeptide + CoA | - |
? | |
2.3.1.97 | myristoyl-CoA + glycylpeptide | enzyme is possibly regulated by calpains in vivo | Bos taurus | N-myristoylglycylpeptide + CoA | - |
? | |
2.3.1.97 | myristoyl-CoA + M2 gene segment of reovirus type 3-derived peptide | - |
Bos taurus | N-myristoylated M2 gene segment of reovirus type 3-derived peptide + CoA | - |
? | |
2.3.1.97 | palmitoyl-CoA + glycylpeptide | poor substrate | Bos taurus | N-palmitoylglycylpeptide + CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.97 | monomer | 1 * 50000, recombinant enzyme | Bos taurus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.97 | NMT | - |
Bos taurus |