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Literature summary extracted from

  • Raju, R.V.S.; Datla, R.S.S.; Sharma, R.K.
    Expression of human N-myristoyltransferase in Escherichia coli. Comparison with N-myristoyltransferases expressed in different tissues (1996), Mol. Cell. Biochem., 155, 69-76.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.97 acetonitrile 2.5fold activation at 5% v/v Homo sapiens
2.3.1.97 ethanol 5fold activation at 10% v/v Homo sapiens
2.3.1.97 SDS 3.2fold activation at 1.73 mM Homo sapiens
2.3.1.97 Tris activates about 2.5fold at 225 mM compared to 40 mM Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.97 construction of expression plasmid encoding the enzyme, lacking the first 9 amino acid residues, fused to a enterokinase cleavage site and a polyhistidine tag, overexpression in Escherichia coli DH5alpha Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.97 factor NIP71 bovine brain inhibitor protein; strong inhibition in a concentration dependent manner, noncompetitive Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.97 49000
-
 1 * 49000, tag cleaved off by enterokinase, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.97 Homo sapiens
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.3.1.97 acetonitrile, stable in 5% v/v Homo sapiens
2.3.1.97 ethanol, stable in 10% v/v Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.97 recombinant from Escherichia coli Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.97 0.248
-
 purified wild-type enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.97 myristoyl-CoA + cAMP-dependent protein kinase-derived peptide
-
 Homo sapiens N-myristoylated cAMP-dependent protein kinase-derived peptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Arg pp60src-derived peptide Homo sapiens N-myristoyl-Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Arg + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide specific for Gly at N-terminus Homo sapiens N-myristoylglycylpeptide + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.97 monomer 1 * 49000, tag cleaved off by enterokinase, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.3.1.97 NMT
-
 Homo sapiens