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Literature summary extracted from

  • Wiegand, R.C.; Carr, C.; Minnerly, J.C.; Pauley, A.M.; Carron, C.P.; Langner, C.A.; Duronio, R.J.; Gordon, J.I.
    The Candida albicans myristoyl-CoA:protein N-myristoyltransferase gene. Isolation and expression in Saccharomyces cerevisiae and Escherichia coli (1992), J. Biol. Chem., 267, 8591-8598.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.97 medicine target for antiviral and antifungal therapy Candida albicans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.97 expression in Escherichia coli, DNA sequence determination Candida albicans

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.97 additional information
-
 Candida albicans
2.3.1.97 additional information temperature sensitive mutant strain nmt1-181, exchange of Gly to Asp, 10fold increased Km for myristoyl-CoA at 36°C Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.97 0.00023
-
 Gly-Asn-Ala-Ala-Ser-Ala-Arg-Arg
-
 Saccharomyces cerevisiae
2.3.1.97 0.0044
-
 Gly-L-Ala-L-Arg-L-Ala-L-Ser-L-Val-L-Leu-L-Ser
-
 Saccharomyces cerevisiae
2.3.1.97 0.006
-
 Gly-Asn-Ala-Ala-Ser-Ala-Arg-Arg
-
 Candida albicans
2.3.1.97 0.017
-
 Gly-Ala-Arg-Ala-Ser-Val-Leu-Ser
-
 Candida albicans
2.3.1.97 0.03
-
 Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae
2.3.1.97 0.6
-
 Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg
-
 Candida albicans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.97 additional information
-
 amino acid sequence, alignment Saccharomyces cerevisiae
2.3.1.97 additional information
-
 amino acid sequence, alignment Candida albicans
2.3.1.97 51880
-
 calculation from gene sequence Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.97 myristoyl-CoA + glycylpeptide Saccharomyces cerevisiae
-
 N-myristoylglycylpeptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide Candida albicans
-
 N-myristoylglycylpeptide + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.97 Candida albicans
-
 single copy gene nmt1
-
2.3.1.97 Candida albicans
-
 strain ATCC 32354
-
2.3.1.97 Saccharomyces cerevisiae
-
 gene nmt1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.97 native enzyme, and recombinant enzyme from Escherichia coli Candida albicans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.97 additional information
-
-
 Saccharomyces cerevisiae
2.3.1.97 additional information
-
-
 Candida albicans
2.3.1.97 0.000024
-
 purified native enzyme Candida albicans
2.3.1.97 0.000149
-
 purified recombinant enzyme Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.97 myristoyl-CoA + Gly-Ala-Arg-Ala-Ser-Val-Leu-Ser
-
 Candida albicans N-myristoyl-Gly-Ala-Arg-Ala-Ser-Val-Leu-Ser + CoA
-
 ?
2.3.1.97 myristoyl-CoA + Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg
-
 Saccharomyces cerevisiae N-myristoyl-Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg + CoA
-
 ?
2.3.1.97 myristoyl-CoA + Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg
-
 Candida albicans N-myristoyl-Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg + CoA
-
 ?
2.3.1.97 myristoyl-CoA + Gly-Asn-Ala-Ala-Ser-Ala-Arg-Arg
-
 Saccharomyces cerevisiae N-myristoyl-Gly-Asn-Ala-Ala-Ser-Ala-Arg-Arg + CoA
-
 ?
2.3.1.97 myristoyl-CoA + Gly-Asn-Ala-Ala-Ser-Ala-Arg-Arg
-
 Candida albicans N-myristoyl-Gly-Asn-Ala-Ala-Ser-Ala-Arg-Arg + CoA
-
 ?
2.3.1.97 myristoyl-CoA + Gly-L-Ala-L-Arg-L-Ala-L-Ser-L-Val-L-Leu-L-Ser
-
 Saccharomyces cerevisiae N-myristoyl-Gly-L-Ala-L-Arg-L-Ala-L-Ser-L-Val-L-Leu-L-Ser + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide
-
 Saccharomyces cerevisiae N-myristoylglycylpeptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide
-
 Candida albicans N-myristoylglycylpeptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide overview peptides and proteins Saccharomyces cerevisiae N-myristoylglycylpeptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide overview peptides and proteins Candida albicans N-myristoylglycylpeptide + CoA
-
 ?
2.3.1.97 palmitoyl-CoA + glycylpeptide no activity Candida albicans N-palmitoylglycylpeptide + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.97 NMT
-
 Saccharomyces cerevisiae
2.3.1.97 NMT
-
 Candida albicans