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Literature summary extracted from

  • Schöppner, A.; Kindl, H.
    Purification and properties of a stilbene synthase from induced cell suspension cultures of peanut (1984), J. Biol. Chem., 259, 6806-6811.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.95 mercuribenzoate inactivation, reactivation by excess of dithiothreitol Arachis hypogaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.95 0.002
-
 4-coumaroyl-CoA
-
 Arachis hypogaea
2.3.1.95 0.01
-
 malonyl-CoA
-
 Arachis hypogaea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.95 45000
-
 2 * 45000, SDS-PAGE Arachis hypogaea
2.3.1.95 90000
-
 gel filtration, sucrose density gradient centrifugation Arachis hypogaea

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.95 Arachis hypogaea
-
 peanut
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.95 ammonium sulfate, DE52 cellulose, hydroxylapatite Arachis hypogaea

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.95 3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + trans-resveratrol + 4 CO2 proposed mechanism Arachis hypogaea
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.95 cell suspension culture
-
 Arachis hypogaea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.95 0.0035
-
-
 Arachis hypogaea

Storage Stability

EC Number Storage Stability Organism
2.3.1.95 -20°C, 1 mM dithiothreitol, 10% sucrose, 3 weeks, 30% loss of activity Arachis hypogaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.95 3-coumaroyl-CoA + malonyl-CoA 9% of activity with 4-coumaroyl-CoA Arachis hypogaea 3,3',5-trihydroxystilbene + CoA + CO2
-
 ?
2.3.1.95 4-coumaroyl-CoA + malonyl-CoA malonyl-CoA cannot be replaced by related compounds e.g. acetyl-CoA Arachis hypogaea 3,4',5-trihydroxystilbene + CoA + CO2 trivial name resveratrol ?
2.3.1.95 caffeoyl-CoA + malonyl-CoA 8% of activity with 4-coumaroyl-CoA Arachis hypogaea 3,3',4',5-tetrahydroxystilbene + CoA + CO2
-
 ?
2.3.1.95 cinnamoyl-CoA + malonyl-CoA 10% of activity with 4-coumaroyl-CoA Arachis hypogaea 3,5-dihydroxystilbene + CoA + CO2
-
 ?
2.3.1.95 dihydro-4-coumaroyl-CoA + malonyl-CoA 13% of activity with 4-coumaroyl-CoA Arachis hypogaea 3,4',5-trihydroxybibenzyl + CoA + CO2
-
 ?
2.3.1.95 feruloyl-CoA + malonyl-CoA 6% of activity with 4-coumaroyl-CoA Arachis hypogaea 3,4',5-trihydroxy-3'-methoxystilbene + CoA + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.95 dimer 2 * 45000, SDS-PAGE Arachis hypogaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.95 7.5 8.5
-
 Arachis hypogaea