EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.34 | expression in Escherichia coli strain DH5alpha as His-tagged enzyme | Enterococcus faecalis |
2.3.1.9 | expression in Escherichia coli | Enterococcus faecalis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.9 | CoA | - |
Enterococcus faecalis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.34 | 0.02 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA | pH 6.5, 37°C | Enterococcus faecalis | |
1.1.1.34 | 0.03 | - |
NADPH | pH 6.5, 37°C, (S)-3-hydroxy-3-methylglutaryl-CoA reduction | Enterococcus faecalis | |
1.1.1.34 | 0.22 | - |
CoA | pH 6.5, 37°C, mevaldehyde oxidation | Enterococcus faecalis | |
1.1.1.34 | 0.23 | - |
CoA | pH 6.5, 37°C, mevalonate oxidation | Enterococcus faecalis | |
1.1.1.34 | 0.25 | - |
NADPH | pH 6.5, 37°C, mevaldehyde reduction | Enterococcus faecalis | |
1.1.1.34 | 0.25 | - |
NADP+ | pH 6.5, 37°C, mevalonate oxidation | Enterococcus faecalis | |
1.1.1.34 | 0.66 | - |
mevaldehyde | pH 6.5, 37°C, oxidation | Enterococcus faecalis | |
1.1.1.34 | 0.67 | - |
NADP+ | pH 6.5, 37°C, mevaldehyde oxidation | Enterococcus faecalis | |
1.1.1.34 | 1 | - |
(R)-mevalonate | pH 6.5, 37°C, oxidation | Enterococcus faecalis | |
1.1.1.34 | 3.8 | - |
mevaldehyde | pH 6.5, 37°C, reduction | Enterococcus faecalis | |
2.3.1.9 | 0.01 | - |
CoA | thiolysis | Enterococcus faecalis | |
2.3.1.9 | 0.088 | - |
acetoacetyl-CoA | thiolysis | Enterococcus faecalis | |
2.3.1.9 | 0.6 | - |
acetyl-CoA | synthesis | Enterococcus faecalis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.9 | 86496 | - |
x * 86496, deduced from nucleotide sequence | Enterococcus faecalis |
2.3.1.9 | 88712 | - |
x * 88712, MALDI-mass spectrometry | Enterococcus faecalis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.34 | (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | Enterococcus faecalis | involved in isopentenyl diphosphate biosynthesis, mevalonate pathway overview | (R)-mevalonate + CoA + 2 NADP+ | - |
r | |
1.1.1.34 | acetyl-CoA | Enterococcus faecalis | - |
acetoacetyl-CoA + CoA | - |
r | |
2.3.1.9 | CoA + acetoacetyl-CoA | Enterococcus faecalis | - |
acetyl-CoA + acetyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.34 | Enterococcus faecalis | - |
dual-function enzyme with acetoacetyl-coenzyme A thiolase and 3-hydroxy-3-methylglutaryl-coenzyme A reductase activities | - |
2.3.1.9 | Enterococcus faecalis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.34 | recombinant His-tagged enzyme from Escherichia coli by nickel-affinity chromatography | Enterococcus faecalis |
2.3.1.9 | recombinant His-tagged thiolase, Ni-affinity column | Enterococcus faecalis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.34 | (R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | via mevaldehyde as an intermediate product | Enterococcus faecalis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.34 | (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | involved in isopentenyl diphosphate biosynthesis, mevalonate pathway overview | Enterococcus faecalis | (R)-mevalonate + CoA + 2 NADP+ | - |
r | |
1.1.1.34 | (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | overall reaction | Enterococcus faecalis | (R)-mevalonate + CoA + 2 NADP+ | - |
r | |
1.1.1.34 | (S)-3-hydroxy-3-methylglutaryl-CoA + NADPH + H+ | first step reaction | Enterococcus faecalis | mevaldehyde + CoA + NADP+ | - |
r | |
1.1.1.34 | acetyl-CoA | - |
Enterococcus faecalis | acetoacetyl-CoA + CoA | - |
r | |
1.1.1.34 | mevaldehyde + NADPH + H+ | second step reaction | Enterococcus faecalis | (R)-mevalonate + NADP+ | - |
r | |
2.3.1.9 | CoA + acetoacetyl-CoA | - |
Enterococcus faecalis | acetyl-CoA + acetyl-CoA | - |
? | |
2.3.1.9 | CoA + acetoacetyl-CoA | enzyme has both thiolase and 3-hydroxy-3-methylglutaryl-CoA reductase activity | Enterococcus faecalis | acetyl-CoA + acetyl-CoA | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.9 | ? | x * 88712, MALDI-mass spectrometry | Enterococcus faecalis |
2.3.1.9 | ? | x * 86496, deduced from nucleotide sequence | Enterococcus faecalis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.34 | acetoacetyl-coenzyme A thiolase/3-hydroxy-3-methylglutaryl-coenzyme A reductase | - |
Enterococcus faecalis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.34 | 37 | - |
about, acetoacetyl-coenzyme A synthesis and thiolysis | Enterococcus faecalis |
2.3.1.9 | 37 | - |
acetoacetyl-CoA synthesis and thiolysis | Enterococcus faecalis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.34 | 6.5 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA reduction | Enterococcus faecalis |
1.1.1.34 | 7 | - |
(R)-mevalonate oxidation | Enterococcus faecalis |
1.1.1.34 | 9.5 | - |
acetoacetyl-coenzyme A thiolysis | Enterococcus faecalis |
1.1.1.34 | 10.5 | - |
acetoacetyl-coenzyme A synthesis | Enterococcus faecalis |
2.3.1.9 | 9.5 | - |
thiolysis | Enterococcus faecalis |
2.3.1.9 | 10.5 | - |
synthesis | Enterococcus faecalis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.9 | 0.12 | - |
CoA | - |
Enterococcus faecalis |