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Literature summary extracted from

  • Kursula, P.; Ojala, J.; Lambeir, A.M.; Wierenga, R.K.
    The catalytic cycle of biosynthetic thiolase: a conformational journey of an acetyl group through four binding modes and two oxyanion holes (2002), Biochemistry, 41, 15543-15556.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.9
-
 Zoogloea ramigera

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.9 wild-type thiolase and acetylated thiolase complexed with CoA, C89A mutant thiolase complexed with acetyl-CoA and acetoacetyl-CoA, Q64A mutant thiolase Zoogloea ramigera

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.9 C89A no thiolytic activity towards acetoacetyl-CoA Zoogloea ramigera
2.3.1.9 Q64A 30% lower kcat than that of the wild-type Zoogloea ramigera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA Zoogloea ramigera
-
 acetyl-CoA + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Zoogloea ramigera P07097
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.9 2 acetyl-CoA = CoA + acetoacetyl-CoA reaction involves 2 chemically distinct steps: acetyl group transfer from acetyl-CoA to Cys89, and transfer of this acetyl group to a second acetyl-CoA in the Claisen condensation step to form acetoacetyl-CoA Zoogloea ramigera
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA
-
 Zoogloea ramigera acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.9 CoA + acetoacetyl-CoA
-
 Zoogloea ramigera acetyl-CoA + acetyl-CoA
-
 r