Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Modis, Y.; Wierenga, R.K.
    A biosynthetic thiolase in complex with a reaction intermediate: the crystal structure provides new insights into the catalytic mechanism (1999), Structure, 7, 1279-1290.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.9 expression in Escherichia coli Zoogloea ramigera

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.9 hangig-drop vapor diffusion at 21°C, crystal structure at 2.0 A resolution Zoogloea ramigera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.9 40495
-
 4 * 40495, electrospray ionization mass spectroscopy Zoogloea ramigera
2.3.1.9 152000
-
 analytical ultracentrifugation Zoogloea ramigera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA Zoogloea ramigera
-
 acetyl-CoA + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Zoogloea ramigera
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.9 2 acetyl-CoA = CoA + acetoacetyl-CoA proposed two-step ping pong mechanism Zoogloea ramigera
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA
-
 Zoogloea ramigera acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.9 CoA + acetoacetyl-CoA
-
 Zoogloea ramigera acetyl-CoA + acetyl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.9 tetramer 4 * 40495, electrospray ionization mass spectroscopy Zoogloea ramigera

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.9 620
-
 acetoacetyl-CoA
-
 Zoogloea ramigera