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Literature summary extracted from

  • Suzuki, F.; Zahler, W.L.; Emerich, D.W.
    Acetoacetyl-CoA thiolase of Bradyrhizobium japonicum bacteroids: purification and properties (1987), Arch. Biochem. Biophys., 254, 272-281.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.9 dithiothreitol stabilizes Bradyrhizobium japonicum
2.3.1.9 glycerol stabilizes Bradyrhizobium japonicum
2.3.1.9 unstable in dilute solution, less than 0.5 mg/ml protein Bradyrhizobium japonicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.9 iodoacetamide 0.5 mM, 96% inhibition, acetoacetyl-CoA partly protects Bradyrhizobium japonicum
2.3.1.9 iodoacetate
-
 Bradyrhizobium japonicum
2.3.1.9 Mg2+ 60 mM, 45% inhibition of thiolysis, 69% inhibition of synthesis Bradyrhizobium japonicum
2.3.1.9 NADH inhibition of thiolysis Bradyrhizobium japonicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.9 0.019
-
 acetoacetyl-CoA
-
 Bradyrhizobium japonicum
2.3.1.9 0.03
-
 CoA
-
 Bradyrhizobium japonicum
2.3.1.9 0.104
-
 acetyl-CoA
-
 Bradyrhizobium japonicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.9 44000
-
 4 * 44000, SDS-PAGE Bradyrhizobium japonicum
2.3.1.9 180000
-
 gel filtration Bradyrhizobium japonicum
2.3.1.9 193000
-
 native PAGE Bradyrhizobium japonicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA Bradyrhizobium japonicum
-
 acetyl-CoA + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Bradyrhizobium japonicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.9 ammonium sulfate, hydroxylapatite, phosphocellulose Bradyrhizobium japonicum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.9 2 acetyl-CoA = CoA + acetoacetyl-CoA ping-pong mechanism Bradyrhizobium japonicum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.9 bacteroid
-
 Bradyrhizobium japonicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.9 102.9
-
-
 Bradyrhizobium japonicum

Storage Stability

EC Number Storage Stability Organism
2.3.1.9 -20°C, in dilute solution, less than 0.5 mg/ml protein, 24 h, complete loss of activity Bradyrhizobium japonicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA
-
 Bradyrhizobium japonicum acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.9 CoA + acetoacetyl-CoA specific for acetoacetyl-CoA, no activity with ketodecanoyl-CoA, dithiothreitol and 2-mercaptoethanol Bradyrhizobium japonicum acetyl-CoA + acetyl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.9 tetramer 4 * 44000, SDS-PAGE Bradyrhizobium japonicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.9 7
-
 condensation Bradyrhizobium japonicum
2.3.1.9 7.8
-
 thiolysis Bradyrhizobium japonicum