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Literature summary extracted from

  • Wiesenborn, D.P.; Rudolph, F.B.; Papoutsakis, E.T.
    Thiolase from Clostridium acetobutylicum ATCC 824 and its role in the synthesis of acids and solvents (1988), Appl. Environ. Microbiol., 54, 2717-2722.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.9 ATP 10 mM, 41% inhibition Clostridium acetobutylicum
2.3.1.9 butyryl-CoA 1 mM, 42% inhibition Clostridium acetobutylicum
2.3.1.9 CoA inhibition in condensation direction Clostridium acetobutylicum
2.3.1.9 Dithionitrobenzoate low but significant inhibition Clostridium acetobutylicum
2.3.1.9 iodoacetamide 0.3 mM, 93% inhibition after 30 min Clostridium acetobutylicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.9 0.0048
-
 CoA
-
 Clostridium acetobutylicum
2.3.1.9 0.032
-
 acetoacetyl-CoA
-
 Clostridium acetobutylicum
2.3.1.9 0.27
-
 acetyl-CoA
-
 Clostridium acetobutylicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.9 44000
-
 4 * 44000, SDS-PAGE Clostridium acetobutylicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA Clostridium acetobutylicum
-
 acetyl-CoA + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Clostridium acetobutylicum
-
 ATCC 824
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.9 ammonium sulfate, DEAE-Sephacel, Blue Sepharose, AX 300 column Clostridium acetobutylicum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.9 2 acetyl-CoA = CoA + acetoacetyl-CoA mechanism Clostridium acetobutylicum
a
2.3.1.9 2 acetyl-CoA = CoA + acetoacetyl-CoA ping-pong mechanism Clostridium acetobutylicum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.9 216
-
-
 Clostridium acetobutylicum

Storage Stability

EC Number Storage Stability Organism
2.3.1.9 4°C, 1 mM dithiothreitol, 10% v/v glycerol, N2 atmosphere, 1 month, less than 10% loss of activity Clostridium acetobutylicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA
-
 Clostridium acetobutylicum acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.9 CoA + acetoacetyl-CoA
-
 Clostridium acetobutylicum acetyl-CoA + acetyl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.9 tetramer 4 * 44000, SDS-PAGE Clostridium acetobutylicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.9 5.5 7.9
-
 Clostridium acetobutylicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.9 5.5 9 69% of maximal activity at pH 9.0 Clostridium acetobutylicum