Literature summary extracted from

Clinkenbeard, K.D.; Sugiyama, T.; Moss, J.; Reed, W.D.; Lane, M.D.
Molecular and catalytic properties of cytosolic acetoacetyl coenzyme A thiolase from avian liver (1973), J. Biol. Chem., 248, 2275-2284.

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.3.1.9	cytosol	-	Gallus gallus	5829	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.3.1.9	41000	-	4 * 41000, SDS-PAGE	Gallus gallus
2.3.1.9	169000	-	sedimentation equilibrium	Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.3.1.9	CoA + acetoacetyl-CoA	Gallus gallus	-	acetyl-CoA + acetyl-CoA	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.3.1.9	Gallus gallus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.3.1.9	cytosolic enzyme	Gallus gallus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.3.1.9	liver	-	Gallus gallus	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.3.1.9	203	-	-	Gallus gallus

Storage Stability

EC Number	Storage Stability	Organism
2.3.1.9	-90°C, 12 months	Gallus gallus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.3.1.9	CoA + acetoacetyl-CoA	-	Gallus gallus	acetyl-CoA + acetyl-CoA	-	?
2.3.1.9	CoA + acetoacetyl-CoA	-	Gallus gallus	acetyl-CoA + acetyl-CoA	-	r

Subunits

EC Number	Subunits	Comment	Organism
2.3.1.9	tetramer	4 * 41000, SDS-PAGE	Gallus gallus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.3.1.9	30	-	assay at	Gallus gallus

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.3.1.9	900	-	thiolytic cleavage	-	Gallus gallus

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Release 2023.1 (January 2023)