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Literature summary extracted from

  • Nishimura, T.; Saito, T.; Tomita, K.
    Purification and properties of beta-ketothiolase from Zoogloea ramigera (1978), Arch. Microbiol., 116, 21-27.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.9 5,5'-dithiobis(2-nitrobenzoate) 0.4 mM, 87% inhibition Zoogloea ramigera
2.3.1.9 iodoacetamide 0.4 mM, 36% inhibition Zoogloea ramigera
2.3.1.9 N-ethylmaleimide 0.4 mM, 53% inhibition Zoogloea ramigera
2.3.1.9 NADH inhibition of thiolysis Zoogloea ramigera
2.3.1.9 NADPH
-
 Zoogloea ramigera
2.3.1.9 p-chloromercuribenzoate 0.4 mM, 95% inhibition Zoogloea ramigera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.9 0.0085
-
 CoA
-
 Zoogloea ramigera
2.3.1.9 0.01
-
 acetoacetyl-CoA
-
 Zoogloea ramigera
2.3.1.9 0.33
-
 acetyl-CoA
-
 Zoogloea ramigera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.9 44000
-
 4 * 44000, SDS-PAGE Zoogloea ramigera
2.3.1.9 190000
-
 gel filtration Zoogloea ramigera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA Zoogloea ramigera
-
 acetyl-CoA + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Zoogloea ramigera
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.9 protamine sulfate, DEAE-cellulose, phosphocellulose, Sephadex G-200 Zoogloea ramigera

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.9 2 acetyl-CoA = CoA + acetoacetyl-CoA bi bi ping pong mechanism Zoogloea ramigera
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.9 91
-
-
 Zoogloea ramigera

Storage Stability

EC Number Storage Stability Organism
2.3.1.9 -20°C, 50 mM Tris-HCl, pH 8.0, 0.5 mM dithiothreitol, glycerol Zoogloea ramigera

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA
-
 Zoogloea ramigera acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.9 CoA + acetoacetyl-CoA
-
 Zoogloea ramigera acetyl-CoA + acetyl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.9 tetramer 4 * 44000, SDS-PAGE Zoogloea ramigera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.9 7.5
-
 condensation Zoogloea ramigera
2.3.1.9 8.5
-
 thiolysis Zoogloea ramigera

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.9 6 9 approx. 80% of maximal activity at pH 6.0, approx. 40% at pH 9.0 Zoogloea ramigera
2.3.1.9 6.5 9.2 approx. 50% of maximal activity at pH 6.5 and pH 9.2, thiolysis Zoogloea ramigera