EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.86 | 3-O-methylmannose | 0.1 mM, lowers Km 4fold, activation by relieving product inhibition by binding long-chain acyl-CoA | Mycolicibacterium phlei | |
2.3.1.86 | 6-O-methylglucose | 0.1 mM, lowers Km 4fold, activation by relieving product inhibition by binding long-chain acyl-CoA | Mycolicibacterium phlei |
EC Number | General Stability | Organism |
---|---|---|
2.3.1.86 | low ionic strength, 0.005 M, leads to dissociation into subunits, partially reactivated by increasing the ionic strength to 0.5 M | Mycolicibacterium phlei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.86 | 0.0096 | - |
malonyl-CoA | in presence of polysaccharide | Mycolicibacterium phlei | |
2.3.1.86 | 0.04 | - |
malonyl-CoA | - |
Mycolicibacterium phlei | |
2.3.1.86 | 0.09 | - |
acetyl-CoA | in presence of polysaccharide | Mycolicibacterium phlei | |
2.3.1.86 | 0.8 | - |
acetyl-CoA | - |
Mycolicibacterium phlei |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.86 | 1390000 | - |
sedimentation velocity data | Mycolicibacterium phlei |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.86 | Mycolicibacterium phlei | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.86 | homogeneity | Mycolicibacterium phlei |
EC Number | Renatured (Comment) | Organism |
---|---|---|
2.3.1.86 | low concentration of phosphate buffer causes dissociation into inactive species, reaggregation and reactivation can be partially achieved by dialysis against 0.5 M phosphate buffer | Mycolicibacterium phlei |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.86 | 0.375 | - |
- |
Mycolicibacterium phlei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.86 | acetyl-CoA + 7 malonyl-CoA + 7 NADH + 7 NADPH + 14 H+ | primers instead of acetyl-CoA: propionyl-CoA, butyryl-CoA or hexanoyl-CoA | Mycolicibacterium phlei | palmitoyl-CoA + 7 CoA + 7 CO2 + 7 NAD+ + 7 NADP+ + 7 H2O | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.86 | NADH | - |
Mycolicibacterium phlei | |
2.3.1.86 | NADPH | requirement | Mycolicibacterium phlei |