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Literature summary extracted from

  • Yein, F.; Brown, O.R.
    Comparative inactivation of yeast fatty acid synthetase component enzymes by 100 atmospheres of oxygen (1977), Biochim. Biophys. Acta, 486, 421-428.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.86 cysteine not Saccharomyces cerevisiae
2.3.1.86 additional information no activation by DTT Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.86 additional information
-
 amino acid composition Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.86 acetyl-CoA + malonyl-CoA + NADH + NADPH Saccharomyces cerevisiae multifunctional enzyme involved in yeast fat metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.86 Saccharomyces cerevisiae
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.3.1.86 under 100 atm nitrogen, at 4°C 1-20 h stable, 40% loss of activity after 40 h Saccharomyces cerevisiae
2.3.1.86 under 100 atm oxygen, at 4°C 1-2 h stable, 48% and 90% loss of activity after 20 h and 40 h, respectively, DTT does not restore activity Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.86 900fold Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.86 additional information
-
 specific activities of components Saccharomyces cerevisiae
2.3.1.86 0.062
-
 substrates: acetoacetyl-cysteamine, malonyl-CoA and NADPH Saccharomyces cerevisiae
2.3.1.86 0.8 0.83 malonyl-CoA Saccharomyces cerevisiae
2.3.1.86 1.6
-
 substrates: acetoacetyl-CoA, malonyl-CoA and NADPH H Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.86 acetyl-CoA + 7 malonyl-CoA + 7 NADH + 7 NADPH + 14 H+
-
 Saccharomyces cerevisiae palmitoyl-CoA + 7 CoA + 7 CO2 + 7 NAD+ + 7 NADP+ + 7 H2O
-
 ?
2.3.1.86 acetyl-CoA + malonyl-CoA + NADH + NADPH multifunctional enzyme involved in yeast fat metabolism Saccharomyces cerevisiae ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.86 37
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.86 NADPH requirement Saccharomyces cerevisiae