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Literature summary extracted from

  • Wang, F.; Wang, X.; Liu, Y.; Tian, W.X.; Zhou, H.M.
    Inhibitive effect of zinc ion on fatty acid synthase from chicken liver (2003), Int. J. Biochem. Cell Biol., 35, 391-400.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.85 Zn2+ 80% loss of activity at 0.008 mM, interacts with SH groups, substrates of the reaction protect, malonyl-CoA being the most effective, addition of dithiothreitol leads to a recovery of 70% enzyme activity; below 0.004 mM, rapid and irreversible inactivation, above 0.004 mM, cross-linking of enzyme involving phosphopantheine SH group. All three substrates, acetyl-CoA, malonyl-CoA, NADPH, protect. Renaturation by dithiothreitol Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.85 270000
-
 subunit, SDS-PAGE Gallus gallus
2.3.1.85 400000
-
 value above 400000 Da, polymer, SDS-PAGE Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.85 acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ Gallus gallus participates in energy metabolism in vivo which is related to adiposis and cancer palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.85 Gallus gallus
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.85 complete renaturation of enzyme inactivated by Zn2+ using dithiothreitol Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.85 liver
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.85 acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+
-
 Gallus gallus palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
-
 ?
2.3.1.85 acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ participates in energy metabolism in vivo which is related to adiposis and cancer Gallus gallus palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
-
 ?