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Literature summary extracted from

  • Jayakumar, A.; Tai, M.H.; Huang, W.Y.; Al-Feel, W.; Hsu, M.; Abu-Elheiga, L.; Chirala, S.S.; Wakil, S.J.
    Human fatty acid synthase: properties and molecular cloning (1995), Proc. Natl. Acad. Sci. USA, 92, 8695-8699.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.85 0.008
-
 acetyl-CoA
-
 Homo sapiens
2.3.1.85 0.02
-
 malonyl-CoA
-
 Homo sapiens
2.3.1.85 0.025
-
 NADPH
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.85 265000
-
 SDS-PAGE Homo sapiens
2.3.1.85 272500
-
 calculated from nucleotide sequence Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.85 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.85 near homogeneity Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.85 brain
-
 Homo sapiens
-
2.3.1.85 liver hepatoma cell line HepG2 Homo sapiens
-
2.3.1.85 lung
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.85 additional information
-
 specific activities of subdomains Homo sapiens
2.3.1.85 0.6
-
 NADPH Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.85 acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ acetoacetyl-CoA can substitute for acetyl-CoA Homo sapiens palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O small amounts of stearate and myristate are also produced ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.85 6.5 6.7
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.85 NADPH
-
 Homo sapiens