BRENDA - Enzyme Database show

Ester formation by alcohol acetyltransferase from brewer's yeast

Yoshioka, K.; Hashimoto, N.; Agric. Biol. Chem. 45, 2183-2190 (1981)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.84
2,4,6-Trinitrobenzenesulfonic acid
-
Saccharomyces uvarum
2.3.1.84
5,5'-dithiobis(2-nitrobenzoate)
99% inhibition
Saccharomyces uvarum
2.3.1.84
Ag+
1 mM, 92% inhibition
Saccharomyces uvarum
2.3.1.84
Cd2+
1 mM, complete inhibition
Saccharomyces uvarum
2.3.1.84
Co2+
1 mM, 83% inhibition
Saccharomyces uvarum
2.3.1.84
Cu2+
1 mM, 99% inhibition
Saccharomyces uvarum
2.3.1.84
ergosterol
; 2 mM, 73% inhibition
Saccharomyces uvarum
2.3.1.84
Hg2+
1 mM, 98% inhibition
Saccharomyces uvarum
2.3.1.84
iodoacetate
-
Saccharomyces uvarum
2.3.1.84
linoleic acid
2 mM, 97% inhibition
Saccharomyces uvarum
2.3.1.84
linolenic acid
2 mM, 97% inhibition
Saccharomyces uvarum
2.3.1.84
monoolein
2 mM, 76% inhibition
Saccharomyces uvarum
2.3.1.84
monostearin
; 2 mM, 58% inhibition
Saccharomyces uvarum
2.3.1.84
Ni2+
1 mM, 68% inhibition
Saccharomyces uvarum
2.3.1.84
oleoyl-CoA
2 mM, 87% inhibition
Saccharomyces uvarum
2.3.1.84
p-chloromercuribenzoate
98% inhibition
Saccharomyces uvarum
2.3.1.84
Pb2+
1 mM, complete inhibition
Saccharomyces uvarum
2.3.1.84
phosphatidylinositol
2 mM, 97% inhibition
Saccharomyces uvarum
2.3.1.84
phosphatidylserine
2 mM, 88% inhibition
Saccharomyces uvarum
2.3.1.84
Tween 60
2 mM, 77% inhibition
Saccharomyces uvarum
2.3.1.84
Tween 80
2 mM, 89% inhibition
Saccharomyces uvarum
2.3.1.84
Zn2+
1 mM, 97% inhibition
Saccharomyces uvarum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.84
membrane
cell membrane bound
Saccharomyces uvarum
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.84
220000
-
gel filtration
Saccharomyces uvarum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.84
Saccharomyces uvarum
-
brewery lager yeast
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.84
Sepharose 6B, DEAE-Sephadex A50, partially purified
Saccharomyces uvarum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.84
0.00729
-
-
Saccharomyces uvarum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.84
butanol + acetyl-CoA
-
487555
Saccharomyces uvarum
butyl acetate + CoA
-
487555
Saccharomyces uvarum
?
2.3.1.84
ethanol + acetyl-CoA
-
487555
Saccharomyces uvarum
ethyl acetate + CoA
-
487555
Saccharomyces uvarum
?
2.3.1.84
ethanol + butyryl-CoA
-
487555
Saccharomyces uvarum
ethyl butyrate + CoA
-
487555
Saccharomyces uvarum
?
2.3.1.84
hexanol + acetyl-CoA
-
487555
Saccharomyces uvarum
hexyl acetate + CoA
-
487555
Saccharomyces uvarum
?
2.3.1.84
isoamylalcohol + acetyl-CoA
-
487555
Saccharomyces uvarum
isoamyl acetate + CoA
-
487555
Saccharomyces uvarum
?
2.3.1.84
methanol + acetyl-CoA
-
487555
Saccharomyces uvarum
methyl acetate + CoA
-
487555
Saccharomyces uvarum
?
2.3.1.84
pentanol + acetyl-CoA
-
487555
Saccharomyces uvarum
pentyl acetate + CoA
-
487555
Saccharomyces uvarum
?
2.3.1.84
propanol + acetyl-CoA
low activity
487555
Saccharomyces uvarum
propyl acetate + CoA
-
487555
Saccharomyces uvarum
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.84
30
-
-
Saccharomyces uvarum
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.3.1.84
10
40
25% of maximal activity at 10°C, 50% of maximal activity at 40°C
Saccharomyces uvarum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.84
7
8
-
Saccharomyces uvarum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.84
5
8.5
approx. 40% of maximal activity at pH 5.0, 80% of maximal activity at pH 8.5
Saccharomyces uvarum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.84
2,4,6-Trinitrobenzenesulfonic acid
-
Saccharomyces uvarum
2.3.1.84
5,5'-dithiobis(2-nitrobenzoate)
99% inhibition
Saccharomyces uvarum
2.3.1.84
Ag+
1 mM, 92% inhibition
Saccharomyces uvarum
2.3.1.84
Cd2+
1 mM, complete inhibition
Saccharomyces uvarum
2.3.1.84
Co2+
1 mM, 83% inhibition
Saccharomyces uvarum
2.3.1.84
Cu2+
1 mM, 99% inhibition
Saccharomyces uvarum
2.3.1.84
ergosterol
; 2 mM, 73% inhibition
Saccharomyces uvarum
2.3.1.84
Hg2+
1 mM, 98% inhibition
Saccharomyces uvarum
2.3.1.84
iodoacetate
-
Saccharomyces uvarum
2.3.1.84
linoleic acid
2 mM, 97% inhibition
Saccharomyces uvarum
2.3.1.84
linolenic acid
2 mM, 97% inhibition
Saccharomyces uvarum
2.3.1.84
monoolein
2 mM, 76% inhibition
Saccharomyces uvarum
2.3.1.84
monostearin
; 2 mM, 58% inhibition
Saccharomyces uvarum
2.3.1.84
Ni2+
1 mM, 68% inhibition
Saccharomyces uvarum
2.3.1.84
oleoyl-CoA
2 mM, 87% inhibition
Saccharomyces uvarum
2.3.1.84
p-chloromercuribenzoate
98% inhibition
Saccharomyces uvarum
2.3.1.84
Pb2+
1 mM, complete inhibition
Saccharomyces uvarum
2.3.1.84
phosphatidylinositol
2 mM, 97% inhibition
Saccharomyces uvarum
2.3.1.84
phosphatidylserine
2 mM, 88% inhibition
Saccharomyces uvarum
2.3.1.84
Tween 60
2 mM, 77% inhibition
Saccharomyces uvarum
2.3.1.84
Tween 80
2 mM, 89% inhibition
Saccharomyces uvarum
2.3.1.84
Zn2+
1 mM, 97% inhibition
Saccharomyces uvarum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.84
membrane
cell membrane bound
Saccharomyces uvarum
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.84
220000
-
gel filtration
Saccharomyces uvarum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.84
Sepharose 6B, DEAE-Sephadex A50, partially purified
Saccharomyces uvarum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.84
0.00729
-
-
Saccharomyces uvarum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.84
butanol + acetyl-CoA
-
487555
Saccharomyces uvarum
butyl acetate + CoA
-
487555
Saccharomyces uvarum
?
2.3.1.84
ethanol + acetyl-CoA
-
487555
Saccharomyces uvarum
ethyl acetate + CoA
-
487555
Saccharomyces uvarum
?
2.3.1.84
ethanol + butyryl-CoA
-
487555
Saccharomyces uvarum
ethyl butyrate + CoA
-
487555
Saccharomyces uvarum
?
2.3.1.84
hexanol + acetyl-CoA
-
487555
Saccharomyces uvarum
hexyl acetate + CoA
-
487555
Saccharomyces uvarum
?
2.3.1.84
isoamylalcohol + acetyl-CoA
-
487555
Saccharomyces uvarum
isoamyl acetate + CoA
-
487555
Saccharomyces uvarum
?
2.3.1.84
methanol + acetyl-CoA
-
487555
Saccharomyces uvarum
methyl acetate + CoA
-
487555
Saccharomyces uvarum
?
2.3.1.84
pentanol + acetyl-CoA
-
487555
Saccharomyces uvarum
pentyl acetate + CoA
-
487555
Saccharomyces uvarum
?
2.3.1.84
propanol + acetyl-CoA
low activity
487555
Saccharomyces uvarum
propyl acetate + CoA
-
487555
Saccharomyces uvarum
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.84
30
-
-
Saccharomyces uvarum
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.3.1.84
10
40
25% of maximal activity at 10°C, 50% of maximal activity at 40°C
Saccharomyces uvarum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.84
7
8
-
Saccharomyces uvarum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.84
5
8.5
approx. 40% of maximal activity at pH 5.0, 80% of maximal activity at pH 8.5
Saccharomyces uvarum