EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.8 | R133A | altered kinetic properties, increased Km for CoA | Methanosarcina thermophila |
2.3.1.8 | R133E | altered kinetic properties, increased Km for CoA | Methanosarcina thermophila |
2.3.1.8 | R133K | altered kinetic properties, increased Km for CoA | Methanosarcina thermophila |
2.3.1.8 | R133Q | altered kinetic properties, increased Km for CoA | Methanosarcina thermophila |
2.3.1.8 | R87A | altered kinetic properties, increased Km for CoA | Methanosarcina thermophila |
2.3.1.8 | R87E | altered kinetic properties, increased Km for CoA | Methanosarcina thermophila |
2.3.1.8 | R87K | altered kinetic properties, increased Km for CoA | Methanosarcina thermophila |
2.3.1.8 | R87Q | altered kinetic properties, increased Km for CoA | Methanosarcina thermophila |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.8 | 2,3-Butanedione | almost complete loss of wild-type enzyme activity after 10 min at 10 mM | Methanosarcina thermophila | |
2.3.1.8 | desulfo-CoA | strong competitive inhibitor | Methanosarcina thermophila | |
2.3.1.8 | phosphate | - |
Methanosarcina thermophila |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.8 | 0.07 | - |
CoA | wild-type | Methanosarcina thermophila | |
2.3.1.8 | 0.11 | - |
CoA | R133K mutant | Methanosarcina thermophila | |
2.3.1.8 | 0.27 | - |
CoA | R87E mutant | Methanosarcina thermophila | |
2.3.1.8 | 0.36 | - |
CoA | R133A mutant | Methanosarcina thermophila | |
2.3.1.8 | 0.7 | - |
CoA | R133Q mutant | Methanosarcina thermophila | |
2.3.1.8 | 0.7 | - |
CoA | R133E mutant | Methanosarcina thermophila | |
2.3.1.8 | 0.75 | - |
CoA | R87K mutant | Methanosarcina thermophila | |
2.3.1.8 | 0.8 | - |
desulfo-CoA | R87E mutant | Methanosarcina thermophila | |
2.3.1.8 | 0.8 | - |
desulfo-CoA | R133K mutant | Methanosarcina thermophila | |
2.3.1.8 | 0.9 | - |
CoA | R87A mutant | Methanosarcina thermophila | |
2.3.1.8 | 1 | - |
desulfo-CoA | R133E mutant | Methanosarcina thermophila | |
2.3.1.8 | 1.1 | - |
CoA | R87Q mutant | Methanosarcina thermophila | |
2.3.1.8 | 1.3 | - |
desulfo-CoA | R87Q mutant | Methanosarcina thermophila | |
2.3.1.8 | 1.3 | - |
desulfo-CoA | R133A mutant | Methanosarcina thermophila | |
2.3.1.8 | 1.4 | - |
desulfo-CoA | wild-type | Methanosarcina thermophila | |
2.3.1.8 | 3.9 | - |
desulfo-CoA | R87K mutant | Methanosarcina thermophila | |
2.3.1.8 | 4 | - |
desulfo-CoA | R133Q mutant | Methanosarcina thermophila | |
2.3.1.8 | 6 | - |
desulfo-CoA | R87A mutant | Methanosarcina thermophila |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.8 | acetyl-CoA + phosphate | Methanosarcina thermophila | - |
CoA + acetyl phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.8 | Methanosarcina thermophila | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.8 | acetyl-CoA + phosphate | - |
Methanosarcina thermophila | CoA + acetyl phosphate | - |
r |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.8 | 0.00467 | - |
desulfo-CoA | R133E mutant | Methanosarcina thermophila | |
2.3.1.8 | 0.0125 | - |
desulfo-CoA | R133A mutant | Methanosarcina thermophila | |
2.3.1.8 | 0.133 | - |
desulfo-CoA | R133K mutant | Methanosarcina thermophila | |
2.3.1.8 | 0.283 | - |
CoA | R87E mutant | Methanosarcina thermophila | |
2.3.1.8 | 0.383 | - |
desulfo-CoA | R87E mutant | Methanosarcina thermophila | |
2.3.1.8 | 0.467 | - |
desulfo-CoA | R133Q mutant | Methanosarcina thermophila | |
2.3.1.8 | 0.683 | - |
desulfo-CoA | R87K mutant | Methanosarcina thermophila | |
2.3.1.8 | 0.733 | - |
CoA | R133E mutant | Methanosarcina thermophila | |
2.3.1.8 | 1.63 | - |
desulfo-CoA | R87Q mutant | Methanosarcina thermophila | |
2.3.1.8 | 3.07 | - |
desulfo-CoA | R87A mutant | Methanosarcina thermophila | |
2.3.1.8 | 4.53 | - |
desulfo-CoA | wild-type | Methanosarcina thermophila | |
2.3.1.8 | 6.22 | - |
CoA | R87K mutant | Methanosarcina thermophila | |
2.3.1.8 | 6.97 | - |
CoA | R133K mutant | Methanosarcina thermophila | |
2.3.1.8 | 10.5 | - |
CoA | R133A mutant | Methanosarcina thermophila | |
2.3.1.8 | 19.1 | - |
CoA | R133Q mutant | Methanosarcina thermophila | |
2.3.1.8 | 20.4 | - |
CoA | R87A mutant | Methanosarcina thermophila | |
2.3.1.8 | 23.2 | - |
CoA | R87Q mutant | Methanosarcina thermophila | |
2.3.1.8 | 81.3 | - |
CoA | wild-type | Methanosarcina thermophila |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.8 | 0.001 | - |
desulfo-CoA | - |
Methanosarcina thermophila | |
2.3.1.8 | 0.32 | - |
phosphate | R133Q mutant | Methanosarcina thermophila | |
2.3.1.8 | 0.52 | - |
phosphate | R87Q mutant | Methanosarcina thermophila | |
2.3.1.8 | 0.81 | - |
phosphate | wild-type enzyme | Methanosarcina thermophila |