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Literature summary extracted from

  • Bock, A.K.; Glasemacher, J.; Schmidt, R.; Schonheit, P.
    Purification and characterization of two extremely thermostable enzymes, phosphate acetyltransferase and acetate kinase, from the hyperthermophilic eubacterium Thermotoga maritima (1999), J. Bacteriol., 181, 1861-1867.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.7.2.1 stabilized against heat inactivation by salts, most effective (NH4)2SO4 Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.8 0.0231
-
 acetyl-CoA
-
 Thermotoga maritima
2.3.1.8 0.024
-
 acetyl phosphate
-
 Thermotoga maritima
2.3.1.8 0.03
-
 CoA
-
 Thermotoga maritima
2.3.1.8 0.111
-
 phosphate
-
 Thermotoga maritima
2.7.2.1 0.44
-
 acetyl phosphate pH 7.0, 50°C Thermotoga maritima
2.7.2.1 0.7
-
 ATP pH 7.0, 50°C Thermotoga maritima
2.7.2.1 3
-
 ADP pH 7.0, 50°C Thermotoga maritima
2.7.2.1 40
-
 acetate pH 7.0, 50°C Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.1 Ca2+
-
 Thermotoga maritima
2.7.2.1 Co2+
-
 Thermotoga maritima
2.7.2.1 Mg2+
-
 Thermotoga maritima
2.7.2.1 Mn2+
-
 Thermotoga maritima
2.7.2.1 Ni2+
-
 Thermotoga maritima
2.7.2.1 Zn2+
-
 Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.8 34000
-
 alpha4, homotetramer, 4 * 34000, SDS-PAGE Thermotoga maritima
2.3.1.8 170000
-
 gel filtration Thermotoga maritima
2.7.2.1 44000
-
 2 * 44000, alpha2 homodimer, SDS-PAGE Thermotoga maritima
2.7.2.1 90000
-
 gel filtration Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate Thermotoga maritima
-
 CoA + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate Thermotoga maritima
-
 ADP + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.8 Thermotoga maritima
-
 hyperthermophilic eubacterium
-
2.7.2.1 Thermotoga maritima
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.7.2.1 not sensitive to oxygen Thermotoga maritima

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.8 apparent homogeneity Thermotoga maritima
2.7.2.1
-
 Thermotoga maritima

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.8 225
-
-
 Thermotoga maritima

Storage Stability

EC Number Storage Stability Organism
2.3.1.8 -20°C, no loss of activity for several weeks Thermotoga maritima
2.7.2.1 -20°C, can be stored in 20 mM Tris-HCl, pH 8.0, 2.0 mM MgCl2, 0.32 M NaCl, supplemented with 10% glycerol, without significant loss of activity for several weeks Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate
-
 Thermotoga maritima CoA + acetyl phosphate
-
 r
2.3.1.8 butyryl-CoA + phosphate
-
 Thermotoga maritima CoA + butyryl phosphate
-
 ?
2.3.1.8 propionyl-CoA + phosphate
-
 Thermotoga maritima CoA + propionyl phosphate
-
 ?
2.7.2.1 ATP + acetate
-
 Thermotoga maritima ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + propionate
-
 Thermotoga maritima ADP + propionyl phosphate
-
 r
2.7.2.1 CTP + acetate
-
 Thermotoga maritima CDP + acetyl phosphate
-
 r
2.7.2.1 GTP + acetate
-
 Thermotoga maritima GDP + acetyl phosphate
-
 r
2.7.2.1 ITP + acetate
-
 Thermotoga maritima IDP + acetyl phosphate
-
 r
2.7.2.1 additional information butyrate, isobutyrate, valerate and isovalerate are no substrates Thermotoga maritima ?
-
 ?
2.7.2.1 UTP + acetate
-
 Thermotoga maritima UDP + acetyl phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.8 tetramer alpha4, homotetramer, 4 * 34000, SDS-PAGE Thermotoga maritima
2.7.2.1 dimer 2 * 44000, alpha2 homodimer, SDS-PAGE Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.8 90
-
-
 Thermotoga maritima
2.7.2.1 90
-
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.2.1 40 100 activity increases rapidly above 55°C, enzyme does not lose activity at 80°C upon incubation for 180 min Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.8 80
-
 stable for 120 min Thermotoga maritima
2.3.1.8 90
-
 60% loss of activity after 120 min Thermotoga maritima
2.3.1.8 100
-
 60% loss of activity after 120 min Thermotoga maritima
2.7.2.1 55 100 almost complete loss about 90% is observed after incubation at 100°C for about 60 min, enzyme does not lose activity upon incubation for 3 h at 100°C in presence of 1 M (NH4)2SO4 Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.1 7
-
-
 Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.2.1 6 8.5 about 50% activity in this range Thermotoga maritima