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Literature summary extracted from

  • Nojiri, T.; Tanaka, F.; Nakayama, I.
    Purification and properties of phosphotransacetylase from Lactobacillus fermenti (1971), J. Biochem., 69, 789-801.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.8 lyophilization causes almost complete loss of enzyme activity Limosilactobacillus fermentum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.8 Ba2+
-
 Limosilactobacillus fermentum
2.3.1.8 Ca2+
-
 Limosilactobacillus fermentum
2.3.1.8 Cs+
-
 Limosilactobacillus fermentum
2.3.1.8 Li+
-
 Limosilactobacillus fermentum
2.3.1.8 Mg2+
-
 Limosilactobacillus fermentum
2.3.1.8 N-ethylmaleimide above 0.1 mM Limosilactobacillus fermentum
2.3.1.8 p-chloromercuribenzoate above 0.1 mM Limosilactobacillus fermentum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.8 0.087
-
 CoA
-
 Limosilactobacillus fermentum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.8 K+ activates Limosilactobacillus fermentum
2.3.1.8 NH4+ activates Limosilactobacillus fermentum
2.3.1.8 Rb+ stimulates Limosilactobacillus fermentum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.8 68000
-
 gel filtration Limosilactobacillus fermentum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate Limosilactobacillus fermentum
-
 CoA + acetyl phosphate
-
 r
2.3.1.8 acetyl-CoA + phosphate Limosilactobacillus fermentum DSM 20391
-
 CoA + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.8 Limosilactobacillus fermentum
-
 IFO 3071
-
2.3.1.8 Limosilactobacillus fermentum DSM 20391
-
 IFO 3071
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.8
-
 Limosilactobacillus fermentum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.8 1124
-
-
 Limosilactobacillus fermentum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.8 3'-dephospho-CoA + acetyl phosphate rate is about one tenth of the activity with CoA Limosilactobacillus fermentum acetyl-3'-dephospho-CoA + phosphate
-
 ?
2.3.1.8 3'-dephospho-CoA + acetyl phosphate rate is about one tenth of the activity with CoA Limosilactobacillus fermentum DSM 20391 acetyl-3'-dephospho-CoA + phosphate
-
 ?
2.3.1.8 acetyl-CoA + phosphate
-
 Limosilactobacillus fermentum CoA + acetyl phosphate
-
 r
2.3.1.8 acetyl-CoA + phosphate
-
 Limosilactobacillus fermentum DSM 20391 CoA + acetyl phosphate
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.8 additional information
-
-
 Limosilactobacillus fermentum
2.3.1.8 45
-
 unstable, can be stabilized by ammonium sulfate Limosilactobacillus fermentum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.8 7.6
-
 Tris buffer Limosilactobacillus fermentum