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Literature summary extracted from

  • Vigenschow, H.; Schwarm, H.M.; Knobloch, K.
    Purification and characterization of a phosphotransacetylase from Rhodopseudomonas palustris (1986), Biol. Chem. Hoppe-Seyler, 367, 957-962.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.8 (NH4)2SO4, Na2SO4, NaCl or KCl stabilizes Rhodopseudomonas palustris
2.3.1.8 ammonium sulfate and potassium phosphate stabilize Rhodopseudomonas palustris
2.3.1.8 ethylene glycol 20% v/v stabilizes Rhodopseudomonas palustris
2.3.1.8 labile in dilute solutions or at elevated temperatures Rhodopseudomonas palustris
2.3.1.8 MgCl2 destabilizes Rhodopseudomonas palustris
2.3.1.8 NH4Cl sligthly stabilizes Rhodopseudomonas palustris
2.3.1.8 repeated freezing and thawing inactivates Rhodopseudomonas palustris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.8 (NH4)2SO4 activation at low concentration, inhibition at high concentration Rhodopseudomonas palustris
2.3.1.8 coenzyme A strong substrate inhibition Rhodopseudomonas palustris
2.3.1.8 LiCl slight Rhodopseudomonas palustris
2.3.1.8 MgCl2 activation at low concentration, inhibition at high concentration Rhodopseudomonas palustris
2.3.1.8 NaCl slight Rhodopseudomonas palustris
2.3.1.8 SO42-
-
 Rhodopseudomonas palustris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.8 0.15
-
 CoA
-
 Rhodopseudomonas palustris
2.3.1.8 4.7
-
 acetyl phosphate
-
 Rhodopseudomonas palustris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.8 K+ activates Rhodopseudomonas palustris
2.3.1.8 K+ KCl activates Rhodopseudomonas palustris
2.3.1.8 NH4+ activates Rhodopseudomonas palustris
2.3.1.8 NH4+ NH4Cl activates Rhodopseudomonas palustris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.8 52500
-
 1 * 52500, SDS-PAGE Rhodopseudomonas palustris
2.3.1.8 54500
-
 gel filtration Rhodopseudomonas palustris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate Rhodopseudomonas palustris
-
 CoA + acetyl phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.8 Rhodopseudomonas palustris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.8
-
 Rhodopseudomonas palustris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.8 31.6
-
-
 Rhodopseudomonas palustris

Storage Stability

EC Number Storage Stability Organism
2.3.1.8 -24°C, (NH4)2SO4, 50-70% loss of activity after some months Rhodopseudomonas palustris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate
-
 Rhodopseudomonas palustris CoA + acetyl phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.8 monomer 1 * 52500, SDS-PAGE Rhodopseudomonas palustris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.8 38
-
-
 Rhodopseudomonas palustris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.8 60
-
 pH 8.0, 50 mM Tris/HCl, complete loss of activity after 1 min Rhodopseudomonas palustris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.8 7.8
-
-
 Rhodopseudomonas palustris