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Literature summary extracted from

  • Kreuzberg, K.; Umlauf, H.; Blaschkowski, H.P.
    Purification and properties of phosphotransacetylase from the eucaryotic green alga Chlorogonium elongatum (1985), Biochim. Biophys. Acta, 842, 22-29.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.3.1.8 stabilized by addition of 200 mM ammonium sulfate and 2-5 mM mercaptans to the extraction buffer Chlorogonium elongatum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.8 acetyl-CoA competitive Chlorogonium elongatum
2.3.1.8 ADP no effect Chlorogonium elongatum
2.3.1.8 ATP no effect Chlorogonium elongatum
2.3.1.8 iodoacetamide 70% inactivation after 1 min at 5 mM Chlorogonium elongatum
2.3.1.8 iodoacetic acid 76% inactivation after 4 min at 5 mM Chlorogonium elongatum
2.3.1.8 phosphate competitive Chlorogonium elongatum
2.3.1.8 S-dimethylarsino-CoA
-
 Chlorogonium elongatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.8 0.078
-
 acetyl-CoA
-
 Chlorogonium elongatum
2.3.1.8 0.09
-
 CoA arsenolysis Chlorogonium elongatum
2.3.1.8 0.093
-
 CoA
-
 Chlorogonium elongatum
2.3.1.8 0.311
-
 acetyl phosphate
-
 Chlorogonium elongatum
2.3.1.8 0.66
-
 acetyl phosphate
-
 Chlorogonium elongatum
2.3.1.8 5.44
-
 phosphate
-
 Chlorogonium elongatum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.8 K+
-
 Chlorogonium elongatum
2.3.1.8 NH4+
-
 Chlorogonium elongatum
2.3.1.8 Tris maximum activity with Tris buffer, with HEPES and MES catalysis is lowered by ca. 15% Chlorogonium elongatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.8 44000
-
 6 * 44000, SDS-PAGE Chlorogonium elongatum
2.3.1.8 280000
-
 gel filtration, SDS-PAGE Chlorogonium elongatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate Chlorogonium elongatum
-
 CoA + acetyl phosphate
-
 r
2.3.1.8 additional information Chlorogonium elongatum physiological function in anaerobic metabolism of eukaryotic green algae rather than in aerobic acetate activation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.8 Chlorogonium elongatum
-
 green alga
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.8
-
 Chlorogonium elongatum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.8 acetyl-CoA + phosphate = CoA + acetyl phosphate rapid equilibrium random bi-bi reaction mechanism Chlorogonium elongatum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.8 additional information
-
 assay methods Chlorogonium elongatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate
-
 Chlorogonium elongatum CoA + acetyl phosphate
-
 r
2.3.1.8 additional information physiological function in anaerobic metabolism of eukaryotic green algae rather than in aerobic acetate activation Chlorogonium elongatum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.8 hexamer 6 * 44000, SDS-PAGE Chlorogonium elongatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.8 28
-
 both directions Chlorogonium elongatum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.8 50
-
 t1/2 18 min Chlorogonium elongatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.8 additional information
-
 maximum activity with Tris buffer, with HEPES and MES catalysis is lowered by ca. 15% Chlorogonium elongatum
2.3.1.8 7.6
-
 both directions Chlorogonium elongatum