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Literature summary extracted from

  • Stadtman, E.R.
    The purification and properties of phosphotransacetylase (1952), J. Biol. Chem., 196, 527-534.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.8 1,3-dimercaptopropanol activates at 0.01 M Clostridium kluyveri
2.3.1.8 cysteine activates at 0.01 M Clostridium kluyveri
2.3.1.8 hydrogen sulfide activates at 0.01 M Clostridium kluyveri
2.3.1.8 thiolglycolic acid activates at 0.01 M Clostridium kluyveri

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.8 citrate buffer 0.1 M, pH 8.0 Clostridium kluyveri
2.3.1.8 Diethylbarbiturate potassium diethylbarbiturate buffer, 0.1 M, pH 8.0 Clostridium kluyveri
2.3.1.8 Li+ 23% inhibition Clostridium kluyveri
2.3.1.8 Na+ 85% inhibition Clostridium kluyveri
2.3.1.8 phosphate inhibition of arsenolysis Clostridium kluyveri
2.3.1.8 potassium diphosphate 0.1 M, pH 8.0 Clostridium kluyveri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.8 K+ activates Clostridium kluyveri
2.3.1.8 K+ rate of arsenolysis increases with increasing potassium or ammonium salt concentration and reaches a maximum rate at 0.02 to 0.03 M salt concentration Clostridium kluyveri
2.3.1.8 NH4+ rate of arsenolysis increases with increasing potassium or ammonium salt concentration and reaches a maximum rate at 0.02 to 0.03 M salt concentration Clostridium kluyveri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate Clostridium kluyveri
-
 CoA + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.8 Clostridium kluyveri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.8
-
 Clostridium kluyveri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.8 950
-
-
 Clostridium kluyveri

Storage Stability

EC Number Storage Stability Organism
2.3.1.8 -20°C, 0.2 M phosphate or Tris-HCl, pH 8.0, several months Clostridium kluyveri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate
-
 Clostridium kluyveri CoA + acetyl phosphate
-
 r
2.3.1.8 arsenate + acetyl-CoA
-
 Clostridium kluyveri acetyl arsenate + CoA
-
 ?
2.3.1.8 additional information arsenolysis Clostridium kluyveri ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.8 7.4 8.2
-
 Clostridium kluyveri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.8 6.8 8.6 pH 6.8: about 25% of activity maximum, pH 8.6: about 90% of activity maximum Clostridium kluyveri