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Literature summary extracted from

  • Brand, B.; Boos, W.
    Maltose transacetylase of Escherichia coli. Mapping and cloning of its structural, gene, mac, and characterization of the enzyme as a dimer of identical polypeptides with a molecular weight of 20,000 (1991), J. Biol. Chem., 266, 14113-14118.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.79 cloning of the structural gene mac Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.79 CoA-SH product inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.79 0.018
-
 acetyl-CoA
-
 Escherichia coli
2.3.1.79 40
-
 triethanolamine
-
 Escherichia coli
2.3.1.79 62
-
 D-glucose
-
 Escherichia coli
2.3.1.79 90
-
 maltose
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.79 20000
-
 2 * 20000, SDS-PAGE Escherichia coli
2.3.1.79 40000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.79 acetyl-CoA + maltose Escherichia coli
-
 CoA + 6-O-acetyl-alpha-D-glucopyranosyl-(1->4)-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.79 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.79
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.79 24.8
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.3.1.79 -70°C, enzyme stored frozen in presence of 0.1% 2-mercaptoethanol and 40% glycerol without appreciable loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.79 acetyl-CoA + D-fructose
-
 Escherichia coli CoA + 6-O-acetyl-D-fructose
-
 ?
2.3.1.79 acetyl-CoA + D-galactose
-
 Escherichia coli CoA + 6-O-acetyl-D-galactose
-
 ?
2.3.1.79 acetyl-CoA + D-glucose
-
 Escherichia coli CoA + acetyl-D-glucose
-
 ?
2.3.1.79 acetyl-CoA + D-mannose
-
 Escherichia coli CoA + 6-O-acetyl-D-mannose
-
 ?
2.3.1.79 acetyl-CoA + maltooligosaccharide
-
 Escherichia coli CoA + ?
-
 ?
2.3.1.79 acetyl-CoA + maltose
-
 Escherichia coli CoA + 6-O-acetyl-alpha-D-glucopyranosyl-(1->4)-D-glucose
-
 ?
2.3.1.79 acetyl-CoA + maltotriose
-
 Escherichia coli CoA + 6-O-acetyl-alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->4)-D-glucopyranose
-
 ?
2.3.1.79 acetyl-CoA + triethanolamine
-
 Escherichia coli CoA + acetyl-triethanolamine
-
 ?
2.3.1.79 additional information rhamnose, arabinose, lactose, isopropyl-1-thio-beta-D-galactoside and trehalose are not substrates Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.79 dimer 2 * 20000, SDS-PAGE Escherichia coli