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Literature summary extracted from

  • Bame, K.J.; Rome, L.H.
    Acetyl coenzyme A:alpha-glucosaminide N-acetyltransferase. Evidence for a transmembrane acetylation mechanism (1985), J. Biol. Chem., 260, 11293-11299.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.78 acetyl-CoA
-
 Rattus norvegicus
2.3.1.78 coenzyme A
-
 Rattus norvegicus
2.3.1.78 additional information iodoacetamide and N-ethylmaleimide are not inhibitory Rattus norvegicus
2.3.1.78 N-acetyl-alpha-D-glucosamine
-
 Rattus norvegicus
2.3.1.78 N-acetyl-beta-D-glucosamine
-
 Rattus norvegicus
2.3.1.78 p-chloromercuribenzoate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.78 0.29 0.3 alpha-D-glucosamine
-
 Rattus norvegicus
2.3.1.78 0.55
-
 acetyl-CoA
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.78 lysosome enzyme is acetylated at the cytoplasmic side of the lysosome and the acetyl group is then transferred to the inside where it is used to acetylate heparan sulfate Rattus norvegicus 5764
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.78 acetyl-CoA + heparan sulfate alpha-D-glucosaminide Rattus norvegicus
-
 CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide
-
 ?
2.3.1.78 additional information Rattus norvegicus enzyme is acetylated at the cytoplasmic side of the lysosome and the acetyl group is then transferred to the inside where it is used to acetylate heparan sulfate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.78 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.78 acetyl-CoA + heparan sulfate alpha-D-glucosaminide = CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide di-iso ping-pong mechanism Rattus norvegicus
a
2.3.1.78 acetyl-CoA + heparan sulfate alpha-D-glucosaminide = CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide transmembranal reaction mechanism Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.78 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.78 acetyl-CoA + alpha-D-glucosamine
-
 Rattus norvegicus CoA + N-acetyl-alpha-D-glucosamine
-
 ?
2.3.1.78 acetyl-CoA + heparan sulfate alpha-D-glucosaminide
-
 Rattus norvegicus CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide
-
 ?
2.3.1.78 acetyl-CoA + heparan sulfate alpha-D-glucosaminide brings about the acetylation of glucosamine groups of heparan sulfate and heparin from which the sulfate has been removed Rattus norvegicus CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide
-
 ?
2.3.1.78 additional information enzyme is acetylated at the cytoplasmic side of the lysosome and the acetyl group is then transferred to the inside where it is used to acetylate heparan sulfate Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.78 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.78 additional information
-
 transmembrane transfer Rattus norvegicus
2.3.1.78 6
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.78 5 5.5 pH 5.0: about 30% of activity maximum, pH 5.5: maximum activity above Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.78 3
-
 coenzyme A versus acetyl-CoA Rattus norvegicus
2.3.1.78 7
-
 coenzyme A versus N-acetyl-alpha-D-glucosamine Rattus norvegicus
2.3.1.78 15
-
 N-acetyl-alpha-D-glucosamine versus glusosamine, no detergents added Rattus norvegicus
2.3.1.78 16.5
-
 N-acetyl-alpha-D-glucosamine versus acetyl-CoA, no detergents added Rattus norvegicus