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Literature summary extracted from

  • Saari, J.C.; Bredberg, D.L.
    Acyl-CoA:retinol acyltransferase and lecithin:retinol acyltransferase activities of bovine retinal pigment epithelial microsomes (1990), Methods Enzymol., 190, 156-163.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.76 medicine retinyl esterification: storage of vitamin A Bos taurus

General Stability

EC Number General Stability Organism
2.3.1.135 all operation involving retinoids are performed at darkness, or under red light Rattus norvegicus
2.3.1.135 all operation involving retinoids are performed at darkness, or under red light Bos taurus
2.3.1.135 buffers are bubbled with argon prior to use Bos taurus
2.3.1.135 dithiothreitol stabilizes Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.135 0.002
-
 all-trans-retinol
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.76 microsome membrane fraction Bos taurus
-
-
2.3.1.135 microsome
-
 Rattus norvegicus
-
-
2.3.1.135 microsome
-
 Bos taurus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.76 acyl-CoA + retinol Bos taurus
-
 CoA + retinyl ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.76 Bos taurus
-
 bovine calf or adult
-
2.3.1.135 Bos taurus
-
-
-
2.3.1.135 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.76 partial purification by differential centrifugation Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.76 neural retina
-
 Bos taurus
-
2.3.1.135 intestinal mucosa
-
 Rattus norvegicus
-
2.3.1.135 liver
-
 Rattus norvegicus
-
2.3.1.135 mammary gland
-
 Rattus norvegicus
-
2.3.1.135 retinal pigment epithelium
-
 Bos taurus
-
2.3.1.135 testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.76 additional information
-
 activity determined from microsomes Bos taurus
2.3.1.135 0.00003
-
 intestinal LRAT Rattus norvegicus
2.3.1.135 0.00006
-
 mammary gland LRAT Rattus norvegicus
2.3.1.135 0.000094
-
 testis LRAT Rattus norvegicus
2.3.1.135 0.000145
-
 liver LRAT Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.3.1.76 -80ºC, Tris-acetate, pH 7, DTT, 6 months Bos taurus
2.3.1.135 -80°C, 10 mM Tris acetate, pH 7, 1 mM dithiothreitol, microsomal fraction, stable for at least 6 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.76 acyl-CoA + retinol
-
 Bos taurus CoA + retinyl ester
-
 ?
2.3.1.76 oleoyl-CoA + retinol
-
 Bos taurus CoA + retinyloleolate
-
 ?
2.3.1.76 palmitoyl-CoA + retinol
-
 Bos taurus CoA + retinylpalmitate
-
 ?
2.3.1.135 additional information little or no ability to transfer acyl groups from lysophosphatidylcholine, phosphatidylethanolamine or phosphatidic acid to retinol-(cellular-retinol-binding protein) Rattus norvegicus ?
-
 ?
2.3.1.135 additional information the fatty acid in the 2-position is important in substrate recognition Rattus norvegicus ?
-
 ?
2.3.1.135 additional information the fatty acid in the 2-position is important in substrate recognition Bos taurus ?
-
 ?
2.3.1.135 phosphatidylcholine + retinol-(cellular-retinol-binding-protein)
-
 Rattus norvegicus 2-acylglycerophosphocholine + retinyl ester-(cellular-retinol-binding-protein)
-
 ?
2.3.1.135 phosphatidylcholine + retinol-(cellular-retinol-binding-protein)
-
 Bos taurus 2-acylglycerophosphocholine + retinyl ester-(cellular-retinol-binding-protein)
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.135 37
-
-
 Rattus norvegicus
2.3.1.135 37
-
 assay at Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.135 37
-
 half-life in absence of substrate: 17 min Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.135 7.5 8
-
 Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.76 8
-
 Tris acetate Bos taurus