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Literature summary extracted from

  • Lardizabal, K.D.; Metz, J.G.; Sakamoto, T.; Hutton, W.C.; Pollard, M.R.; Lassner, M.W.
    Purification of a jojoba embryo wax synthase, cloning of its cDNA, and production of high levels of wax in seeds of transgenic Arabidopsis (2000), Plant Physiol., 122, 645-655.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.75 agriculture transgenic plants expressing the enzyme allow the production of long-chain liquid waxes at reasonable cost for use in commercial applications Simmondsia chinensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.75 cloning of cDNA, DNA sequence analysis, construction of plasmid to express the cDNA in transgenic Arabidopsis thaliana plants Simmondsia chinensis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.75 additional information cloned cDNA is expressed in transgenic Arabidopsis thaliana plants, in combination with cDNAs encoding the jojoba fatty acyl-CoA reductase and a beta-ketoacyl-CoA synthase from Lunaria annua under a seed specific promotor, highly enhanced wax content in transgenic seeds Simmondsia chinensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.75 microsome
-
 Simmondsia chinensis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.75 additional information
-
 amino acid sequence analysis and alignment Simmondsia chinensis
2.3.1.75 30000
-
 x * 30000, SDS-PAGE Simmondsia chinensis
2.3.1.75 40000
-
 gel filtration Simmondsia chinensis

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.75 Simmondsia chinensis Q9XGY6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.75 partially Simmondsia chinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.75 embryo developing Simmondsia chinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.75 eicosenoyl-CoA + octadecenol best substrates Simmondsia chinensis octadecenyl eicosenoate + CoA
-
 ?
2.3.1.75 additional information substrate specificity, overview Simmondsia chinensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.75 ? x * 30000, SDS-PAGE Simmondsia chinensis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.75 fatty acyl-coenzyme A:fatty alcohol acyltransferase
-
 Simmondsia chinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.75 additional information
-
 pI: 9.8 Simmondsia chinensis