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Literature summary extracted from

  • Knogge, W.; Schmelzer, E.; Weissenböck, G.
    The role of chalcone synthase in the regulation of flavonoid biosynthesis in developing oat primary leaves (1986), Arch. Biochem. Biophys., 250, 364-372.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.74 apigenin competitive with respect to 4-coumaroyl-CoA and non competitive with respect to malonyl-CoA Avena sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.74 0.0015
-
 4-coumaroyl-CoA
-
 Avena sativa
2.3.1.74 0.0063
-
 malonyl-CoA
-
 Avena sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.74 4-coumaroyl-CoA + 3 malonyl-CoA Avena sativa first step in flavonoid biosynthesis in plants naringenin chalcone + 4 CoA + 3 CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.74 Avena sativa
-
 oat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.74 500fold Avena sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.74 leaf
-
 Avena sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.74 0.00918
-
-
 Avena sativa

Storage Stability

EC Number Storage Stability Organism
2.3.1.74 -20°C, 3 mg protein/ml Avena sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.74 4-coumaroyl-CoA + 3 malonyl-CoA first step in flavonoid biosynthesis in plants Avena sativa naringenin chalcone + 4 CoA + 3 CO2
-
 ?
2.3.1.74 malonyl-CoA + 4-coumaroyl-CoA
-
 Avena sativa naringenin chalcone + CoA + CO2 due to the presence of isomerase the immediate product naringenin chalcone is not detectable, instead 4,2',4',6'-tetrahydroxychalcone is found, the latter cyclizes spontaneously to naringenin ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.74 8
-
-
 Avena sativa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.74 0.0004
-
 apigenin
-
 Avena sativa