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Literature summary extracted from

  • Hrazdina, G.; Lifson, E.; Weeden, N.F.
    Isolation and characterization of buckwheat (Fagopyrum esculentum M.) chalcone synthase and its polyclonal antibodies (1986), Arch. Biochem. Biophys., 247, 414-419.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.74 0.0006
-
 4-coumaroyl-CoA
-
 Fagopyrum esculentum
2.3.1.74 0.001
-
 malonyl-CoA
-
 Fagopyrum esculentum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.74 41000
-
 2 * 41000, SDS-PAGE Fagopyrum esculentum
2.3.1.74 83000
-
 gel filtration Fagopyrum esculentum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.74 4-coumaroyl-CoA + 3 malonyl-CoA Fagopyrum esculentum
-
 naringenin chalcone + 4 CoA + 3 CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.74 Fagopyrum esculentum
-
 buckwheat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.74 homogeneity Fagopyrum esculentum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.74 hypocotyl
-
 Fagopyrum esculentum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.74 additional information
-
-
 Fagopyrum esculentum

Storage Stability

EC Number Storage Stability Organism
2.3.1.74 -20°C, 10% glycerol, 6 months, 20% loss of activity Fagopyrum esculentum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.74 4-coumaroyl-CoA + 3 malonyl-CoA
-
 Fagopyrum esculentum naringenin chalcone + 4 CoA + 3 CO2
-
 ?
2.3.1.74 caffeoyl-CoA + 3 malonyl-CoA 80% enzyme activity Fagopyrum esculentum eriodictyol + 4 CoA + 3 CO2
-
 ?
2.3.1.74 ferulyl-CoA + malonyl-CoA 80% enzyme activity Fagopyrum esculentum homoeriodictyol + CoA + CO2
-
 ?
2.3.1.74 malonyl-CoA + 4-coumaroyl-CoA
-
 Fagopyrum esculentum naringenin chalcone + CoA + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.74 dimer 2 * 41000, SDS-PAGE Fagopyrum esculentum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.74 8
-
-
 Fagopyrum esculentum