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Literature summary extracted from

  • Peters, A.; Schneider-Poetsch, H.A.W.; Schwarz, H.; Weissenböck, G.
    Biochemical and immunological characterization of chalcone synthase from rye leaves (1988), J. Plant Physiol., 133, 178-182.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.74 apigenin 50% inhibition at 0.009 mM Secale cereale
2.3.1.74 CoA 50% inhibition at 0.036 mM Secale cereale
2.3.1.74 eriodictyol 50% inhibition at 0.045 mM Secale cereale
2.3.1.74 isovitexin 2''-O-arabinoside 50% inhibition at 0.062 mM Secale cereale
2.3.1.74 luteolin 50% inhibition at 0.013 mM Secale cereale
2.3.1.74 naringenin 50% inhibition at 0.045 mM Secale cereale

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.74 0.0006
-
 4-coumaroyl-CoA
-
 Secale cereale
2.3.1.74 0.0014 0.0015 malonyl-CoA
-
 Secale cereale
2.3.1.74 0.00145
-
 caffeoyl-CoA
-
 Secale cereale

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.74 43000
-
 1 * 43000 + 1 * 44000, SDS-PAGE Secale cereale
2.3.1.74 44000
-
 1 * 43000 + 1 * 44000, SDS-PAGE Secale cereale

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.74 Secale cereale
-
 rye
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.74 450fold Secale cereale

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.74 leaf seedling Secale cereale
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.74 0.0064
-
-
 Secale cereale

Storage Stability

EC Number Storage Stability Organism
2.3.1.74 -20°C, 4 mg/ml bovine serum albumin Secale cereale

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.74 caffeoyl-CoA + 3 malonyl-CoA
-
 Secale cereale eriodictyol + 4 CoA + 3 CO2
-
 ?
2.3.1.74 malonyl-CoA + 4-coumaroyl-CoA
-
 Secale cereale naringenin chalcone + CoA + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.74 dimer 1 * 43000 + 1 * 44000, SDS-PAGE Secale cereale

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.74 6.5
-
 caffeoyl-CoA Secale cereale
2.3.1.74 8
-
 4-coumaroyl-CoA Secale cereale