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Literature summary extracted from

  • Garcia, R.E.; Mudd, J.B.
    1,2-Diacyl-sn-glycerol: sterol acyl transferase from spinach leaves (Spinacia olerecea L.) (1981), Methods Enzymol., 71, 768-772.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.73 phosphatidylcholine indispensable for activity Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.73 Digitonin effective inhibitor Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.73 additional information differential centrifugation of the leaf homogenate shows the 20000 * g pellet to have the highest amount of enzyme of the fractions tested but the highest specific activity is in the 88000 * g pellet. The active fractions contain endoplasmic reticulum, ribosomes and other membrane vesicles Spinacia oleracea
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-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.73 Spinacia oleracea
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.73 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.73 0.0002
-
 leaf homogenate Spinacia oleracea
2.3.1.73 0.0014
-
 20000 * g pellet from differential centrifugation of the leaf homogenate Spinacia oleracea
2.3.1.73 0.0022
-
 88000 * g pellet from differential centrifugation of the leaf homogenate Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
2.3.1.73 -15°C, in a desiccator, several weeks Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.73 1,2-diacyl-sn-glycerol + sterol
-
 Spinacia oleracea monoacylglycerol + sterol ester
-
 ?
2.3.1.73 campesterol + 1,2-diacyl-sn-glycerol
-
 Spinacia oleracea monoacylglycerol + campesterol-3-O-acyl ester
-
 ?
2.3.1.73 cholesterol + 1,2-diacyl-sn-glycerol
-
 Spinacia oleracea monoacylglycerol + cholesterol-3-O-acyl ester
-
 ?
2.3.1.73 diacylglycerol + 1,2-diacyl-sn-glycerol
-
 Spinacia oleracea monoacylglycerol + triacyl-sn-glycerol
-
 ?
2.3.1.73 additional information testing of diacylglycerols with only one type of fatty acid, preference for fatty acid transfer Spinacia oleracea ?
-
 ?
2.3.1.73 additional information monoacylglycerol and triacylglycerol are not suitable substrates Spinacia oleracea ?
-
 ?
2.3.1.73 sitosterol + 1,2-diacyl-sn-glycerol
-
 Spinacia oleracea monoacylglycerol + sitosterol-3-O-acyl ester
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.73 6
-
 MES-NaOH buffer Spinacia oleracea