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Literature summary extracted from

  • Miyazawa, S.; Ozasa, H.; Furuta, S.; Osumi, T.; Hashimoto, T.
    Purification and properties of carnitine acetyltransferase from rat liver (1983), J. Biochem., 93, 439-451.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.7 enzyme is stable throughout purification procedure Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.7 4-hydroxynonenal
-
 Rattus norvegicus
2.3.1.7 iodoacetic acid
-
 Rattus norvegicus
2.3.1.7 p-chloromercuriphenyl sulfonic acid
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.7 0.022
-
 CoASH substrate hexanoylcarnitine, enzyme from liver homogenate Rattus norvegicus
2.3.1.7 0.024
-
 CoASH substrate decanoylcarnitine, enzyme from liver homogenate Rattus norvegicus
2.3.1.7 0.027
-
 CoASH substrate decanoylcarnitine, enzyme from mitochondria Rattus norvegicus
2.3.1.7 0.027
-
 CoASH substrate butyrylcarnitine and octanoylcarnitine, enzyme from liver homogenate Rattus norvegicus
2.3.1.7 0.028
-
 CoASH substrate acetylcarnitine, enzyme from liver homogenate Rattus norvegicus
2.3.1.7 0.032
-
 CoASH substrate acetylcarnitine, enzyme from mitochondria Rattus norvegicus
2.3.1.7 0.038
-
 CoASH substrate butyrylcarnitine, enzyme from mitochondria Rattus norvegicus
2.3.1.7 0.044
-
 CoASH substrate hexanoylcarnitine, enzyme from mitochondria Rattus norvegicus
2.3.1.7 0.054
-
 CoASH substrate octanoylcarnitine, enzyme from mitochondria Rattus norvegicus
2.3.1.7 0.39
-
 acetylcarnitine enzyme from liver homogenate Rattus norvegicus
2.3.1.7 0.48
-
 butyrylcarnitine enzyme from liver homogenate Rattus norvegicus
2.3.1.7 0.6
-
 acetylcarnitine enzyme from mitochondria Rattus norvegicus
2.3.1.7 0.64
-
 decanoylcarnitine enzyme from mitochondria Rattus norvegicus
2.3.1.7 0.66
-
 decanoylcarnitine enzyme from liver homogenate Rattus norvegicus
2.3.1.7 0.66
-
 butyrylcarnitine enzyme from mitochondria Rattus norvegicus
2.3.1.7 1.27
-
 octanoylcarnitine enzyme from mitochondria Rattus norvegicus
2.3.1.7 1.62
-
 octanoylcarnitine enzyme from liver homogenate Rattus norvegicus
2.3.1.7 2.28
-
 hexanoylcarnitine enzyme from liver homogenate Rattus norvegicus
2.3.1.7 2.51
-
 hexanoylcarnitine enzyme from mitochondria Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.7 mitochondrion
-
 Rattus norvegicus 5739
-
2.3.1.7 peroxisome
-
 Rattus norvegicus 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.7 25000
-
 1 * 34000 + 1 * 25000, purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate, SDS-PAGE Rattus norvegicus
2.3.1.7 27000
-
 1 * 36500 + 1 * 27000, purified from mitochondrial fraction, monomeric form converted into dimeric by proteolytic modification after disruption of mitochondria, SDS-PAGE Rattus norvegicus
2.3.1.7 34000
-
 1 * 34000 + 1 * 25000, purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate, SDS-PAGE Rattus norvegicus
2.3.1.7 36500
-
 1 * 36500 + 1 * 27000, purified from mitochondrial fraction, monomeric form converted into dimeric by proteolytic modification after disruption of mitochondria, SDS-PAGE Rattus norvegicus
2.3.1.7 56000
-
 purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate Rattus norvegicus
2.3.1.7 61900
-
 purified from mitochondrial fraction, gel filtration Rattus norvegicus
2.3.1.7 66100
-
 purified from liver homogenate, gel filtration Rattus norvegicus
2.3.1.7 67500
-
 purified from liver homogenate, SDS-PAGE Rattus norvegicus
2.3.1.7 69000
-
 translation product of the enzyme by the reticulocyte lysate protein-synthesizing system Rattus norvegicus
2.3.1.7 69000
-
 post-mitochondrial fraction, 2 polypeptides 67500 Da + 69000 Da cannot be separated from each other Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.7 acetyl-CoA + carnitine Rattus norvegicus
-
 O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + carnitine Columba sp.
-
 O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine Rattus norvegicus
-
 acylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine Columba sp.
-
 acylcarnitine + CoASH
-
 r
2.3.1.7 acylcarnitine + CoASH Rattus norvegicus
-
 acyl-CoA + carnitine
-
 r
2.3.1.7 acylcarnitine + CoASH Columba sp.
-
 acyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH Rattus norvegicus
-
 acetyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH Columba sp.
-
 acetyl-CoA + carnitine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.7 Columba sp.
-
 pigeon
-
2.3.1.7 Rattus norvegicus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.7 purified from mitochondrial fraction and from homogenate of frozen liver Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.7 liver
-
 Rattus norvegicus
-
2.3.1.7 liver
-
 Columba sp.
-
2.3.1.7 muscle
-
 Rattus norvegicus
-
2.3.1.7 muscle
-
 Columba sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.7 120
-
 purified from postmitochondrial fraction Rattus norvegicus
2.3.1.7 220
-
 purified from liver homogenate Rattus norvegicus
2.3.1.7 240
-
 purified from mitochondrial fraction Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.3.1.7 -20°C, 50 mM potassium phosphate buffer, pH 7.5, purified enzyme can be stored for several months without loss of activity Rattus norvegicus
2.3.1.7 -20°C, frozen mitochondrial fraction can be stored for several months without loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.7 acetyl-CoA + carnitine
-
 Rattus norvegicus O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + carnitine
-
 Columba sp. O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + L-(-)carnitine
-
 Rattus norvegicus O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + L-(-)carnitine
-
 Columba sp. O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine
-
 Rattus norvegicus acylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine
-
 Columba sp. acylcarnitine + CoASH
-
 r
2.3.1.7 acylcarnitine + CoASH
-
 Rattus norvegicus acyl-CoA + carnitine
-
 r
2.3.1.7 acylcarnitine + CoASH
-
 Columba sp. acyl-CoA + carnitine
-
 r
2.3.1.7 decanoyl-CoA + carnitine
-
 Rattus norvegicus decanoylcarnitine + CoASH
-
 r
2.3.1.7 decanoyl-CoA + carnitine
-
 Columba sp. decanoylcarnitine + CoASH
-
 r
2.3.1.7 decanoylcarnitine + CoASH
-
 Rattus norvegicus decanoyl-CoA + carnitine
-
 r
2.3.1.7 decanoylcarnitine + CoASH
-
 Columba sp. decanoyl-CoA + carnitine
-
 r
2.3.1.7 hexanoyl-CoA + carnitine
-
 Rattus norvegicus hexanoylcarnitine + CoASH
-
 r
2.3.1.7 hexanoyl-CoA + carnitine
-
 Columba sp. hexanoylcarnitine + CoASH
-
 r
2.3.1.7 hexanoylcarnitine + CoASH
-
 Rattus norvegicus hexanoyl-CoA + carnitine
-
 r
2.3.1.7 hexanoylcarnitine + CoASH
-
 Columba sp. hexanoyl-CoA + carnitine
-
 r
2.3.1.7 n-butyryl-CoA + carnitine
-
 Rattus norvegicus n-butyrylcarnitine + CoASH
-
 r
2.3.1.7 n-butyrylcarnitine + CoASH
-
 Rattus norvegicus n-butyryl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Rattus norvegicus acetyl-CoA + L-(-)carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Columba sp. acetyl-CoA + L-(-)carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Rattus norvegicus acetyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Columba sp. acetyl-CoA + carnitine
-
 r
2.3.1.7 octanoyl-CoA + carnitine
-
 Rattus norvegicus octanoylcarnitine + CoASH
-
 r
2.3.1.7 octanoylcarnitine + CoASH
-
 Rattus norvegicus octanoyl-CoA + carnitine
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.7 dimer 1 * 36500 + 1 * 27000, purified from mitochondrial fraction, monomeric form converted into dimeric by proteolytic modification after disruption of mitochondria, SDS-PAGE Rattus norvegicus
2.3.1.7 dimer 1 * 34000 + 1 * 25000, purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.7 4 25 stored in a refrigerator for 2 days or incubated at 25°C for 5 h, monomeric form remains unchanged Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.7 8
-
-
 Rattus norvegicus