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Literature summary extracted from

  • Claus, R.; Kaeppeli, O.; Fiechter, A.
    Purification of carnitine acetyltransferase from Candida tropicalis (1982), Anal. Biochem., 127, 376-379.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.7 analysis specific activity is higher than in commercial preparations from other sources, enzyme prepared from yeast may be used for determination of carnitine in biological materials, this preparation is free of acetyl-CoA-deacetylase activity Candida tropicalis
2.3.1.7 analysis in medicine and biochemical practice, the enzyme isolated from breast muscle is used analytically Columba sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.7 0.13
-
 acetyl-CoA
-
 Candida tropicalis
2.3.1.7 3.3
-
 DL-carnitine
-
 Candida tropicalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.7 60000
-
 SDS-PAGE Candida tropicalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.7 acetyl-CoA + carnitine Candida tropicalis
-
 O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + carnitine Columba sp.
-
 O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine Candida tropicalis
-
 acylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine Columba sp.
-
 acylcarnitine + CoASH
-
 r
2.3.1.7 acylcarnitine + CoASH Candida tropicalis
-
 acyl-CoA + carnitine
-
 r
2.3.1.7 acylcarnitine + CoASH Columba sp.
-
 acyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH Candida tropicalis
-
 acetyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH Columba sp.
-
 acetyl-CoA + carnitine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.7 Candida tropicalis
-
 yeast
-
2.3.1.7 Candida tropicalis
-
 ATCC 32113
-
2.3.1.7 Columba sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.7
-
 Candida tropicalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.7 breast muscle
-
 Columba sp.
-
2.3.1.7 muscle
-
 Columba sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.7 0.021
-
-
 Candida tropicalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.7 acetyl-CoA + carnitine
-
 Candida tropicalis O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + carnitine
-
 Columba sp. O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + L-(-)carnitine
-
 Candida tropicalis O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + L-(-)carnitine
-
 Columba sp. O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine
-
 Candida tropicalis acylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine
-
 Columba sp. acylcarnitine + CoASH
-
 r
2.3.1.7 acylcarnitine + CoASH
-
 Candida tropicalis acyl-CoA + carnitine
-
 r
2.3.1.7 acylcarnitine + CoASH
-
 Columba sp. acyl-CoA + carnitine
-
 r
2.3.1.7 malonyl-CoA + L-(-)carnitine
-
 Candida tropicalis malonylcarnitine + CoASH
-
 r
2.3.1.7 malonylcarnitine + CoASH slight transfer of the acyl group to carnitine Candida tropicalis malonyl-CoA + L-(-)carnitine
-
 r
2.3.1.7 additional information
-
 Columba sp. ?
-
 ?
2.3.1.7 additional information crotonyl-CoA is no substrate Candida tropicalis ?
-
 ?
2.3.1.7 n-butyryl-CoA + carnitine
-
 Candida tropicalis n-butyrylcarnitine + CoASH
-
 r
2.3.1.7 n-butyrylcarnitine + CoASH
-
 Candida tropicalis n-butyryl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Candida tropicalis acetyl-CoA + L-(-)carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Columba sp. acetyl-CoA + L-(-)carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Candida tropicalis acetyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Columba sp. acetyl-CoA + carnitine
-
 r
2.3.1.7 propionyl-CoA + L-(-)carnitine
-
 Candida tropicalis propionylcarnitine + CoASH
-
 r
2.3.1.7 propionylcarnitine + CoASH
-
 Candida tropicalis propionyl-CoA + L-(-)carnitine
-
 r