Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gomez-Cambronero, J.; Velasco, S.; Sanchez-Crespo, M.; Vivanco, F.; Mato, J.M.
    Partial purification and characterization of 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine: acetyl-CoA acetyltransferase from rat spleen (1986), Biochem. J., 237, 439-445.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.3.1.67 stable to lyophilization Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.67 sodium dodecylsulfate inactivation at 0.1% Rattus norvegicus
2.3.1.67 Urea inactivation at 8 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.67 0.137
-
 acetyl-CoA
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.67 microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.67 29000
-
 x * 29000, SDS-PAGE and affinity labelling experiments Rattus norvegicus
2.3.1.67 800000
-
 MW after gel filtration, aggregate of several proteins or protein aggregates coated with lipids Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.67 Canis lupus familiaris
-
-
-
2.3.1.67 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.67 solubilization in sodium deoxycholate, precipitation by ammonium sulfate, ultragel AcA-22 chromatography, DEAE-sepharose CL-6B chromatography, covalent chromatography with thiopropyl-sepharose 6B Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.67 liver
-
 Rattus norvegicus
-
2.3.1.67 liver
-
 Canis lupus familiaris
-
2.3.1.67 spleen
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.67 additional information
-
-
 Rattus norvegicus
2.3.1.67 additional information
-
-
 Canis lupus familiaris
2.3.1.67 0.317
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.67 acetyl-CoA + 1-palmitoyl-2-lyso-sn-glycero-3-phosphocholine 12% of the activity observed in presence of alkyl-lyso-sn-glycero-phosphocholine Rattus norvegicus CoA + 2-acetyl-1-palmitoyl-sn-glycero-3-phosphocholine
-
 ?
2.3.1.67 acetyl-CoA + alkyl-sn-glycero-3-phosphocholine
-
 Rattus norvegicus CoA + 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine
-
 ?
2.3.1.67 oleoyl-CoA + alkyl-lyso-glycero-3-phosphoethanolamine 10% of the activity observed in presence of acetyl-CoA Rattus norvegicus CoA + 1-alkyl-2-oleoyl-glycero-3-phosphoethanolamine
-
 ?
2.3.1.67 palmitoyl-CoA + alkyl-lyso-glycero-3-phosphoethanolamine 5% of the activity observed in presence of acetyl-CoA Rattus norvegicus CoA + acetyl-2-palmitoyl-glycero-3-phosphoethanolamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.67 ? x * 29000, SDS-PAGE and affinity labelling experiments Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.67 8
-
-
 Rattus norvegicus