EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.66 | Mg2+ | weak | Streptomyces roseus | |
2.3.1.66 | Mn2+ | weak | Streptomyces roseus | |
2.3.1.66 | N-ethylmaleimide | strong | Streptomyces roseus | |
2.3.1.66 | p-chloromercuribenzoate | strong | Streptomyces roseus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.66 | 0.005 | - |
acetyl-CoA | - |
Streptomyces roseus | |
2.3.1.66 | 0.06 | - |
L-leucine | - |
Streptomyces roseus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.66 | Cu2+ | slight stimulation | Streptomyces roseus | |
2.3.1.66 | Fe2+ | stimulates strongly | Streptomyces roseus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.66 | 27000 | - |
gel filtration | Streptomyces roseus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.66 | acetyl-CoA + L-leucine | Streptomyces roseus | - |
CoA + N-acetyl-L-leucine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.66 | Streptomyces roseus | - |
strain MA839-A1, also present in strain MA839-A1 LN-S | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.66 | partial | Streptomyces roseus |
EC Number | Storage Stability | Organism |
---|---|---|
2.3.1.66 | -180°C, 3 months, 70% remaining activity | Streptomyces roseus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.66 | acetyl-CoA + L-arginine | - |
Streptomyces roseus | CoA + ? | - |
? | |
2.3.1.66 | acetyl-CoA + L-leucine | - |
Streptomyces roseus | CoA + N-acetyl-L-leucine | - |
? | |
2.3.1.66 | acetyl-CoA + L-phenylalanine | - |
Streptomyces roseus | CoA + N-acetyl-L-phenylalanine | - |
? | |
2.3.1.66 | acetyl-CoA + L-valine | - |
Streptomyces roseus | CoA + N-acetyl-L-valine | - |
? | |
2.3.1.66 | additional information | dipeptides, e.g. L-leucyl-L-leucine, are acetylated more rapidly than L-leucine or peptides with more than two amino acids, D-isomers are acetylated more slowly than L-isomers | Streptomyces roseus | ? | - |
? | |
2.3.1.66 | propionyl-CoA + L-leucine | 10% of activity compared to acetyl-CoA | Streptomyces roseus | CoA + propionyl-L-leucine | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.66 | 27 | - |
assay at | Streptomyces roseus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.66 | 9 | - |
assay at | Streptomyces roseus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.66 | 7 | 9 | 10% of maximal activity at pH 7, maximal activity at pH 9 | Streptomyces roseus |