BRENDA - Enzyme Database show

Purification and characterization of bile acid-CoA:amino acid N-acyltransferase from rat liver

Killenberg, P.G.; Jordan, J.T.; J. Biol. Chem. 253, 1005-1010 (1978)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.65
5,5'-dithiobis(2-nitrobenzoic acid)
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.65
additional information
-
additional information
-
Rattus norvegicus
2.3.1.65
0.8
-
aminomethanesulfonate
+ 0.05 mM deoxycholoyl-CoA
Rattus norvegicus
2.3.1.65
0.8
-
taurine
+ 0.05 mM deoxycholoyl-CoA
Rattus norvegicus
2.3.1.65
31
-
glycine
+ 0.05 mM deoxycholoyl-CoA
Rattus norvegicus
2.3.1.65
175
-
beta-Alanine
-
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.65
choloyl-CoA + taurine
Rattus norvegicus
taurine conjugates predominate in rat bile at normal hepatocellular concentrations of glycine and taurine
CoA + taurocholate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.65
Rattus norvegicus
-
male sprague-dawley rats
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.65
partial, 200fold
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.65
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.3.1.65
additional information
-
-
Rattus norvegicus
2.3.1.65
1.99
-
taurine
Rattus norvegicus
2.3.1.65
3.88
-
glycine
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.65
-70C, several weeks, stable
Rattus norvegicus
2.3.1.65
0 to 20C, several h, stable
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.65
chenodeoxycholoyl-CoA + glycine
-
487368
Rattus norvegicus
CoA + glycochenodeoxycholate
-
-
-
?
2.3.1.65
chenodeoxycholoyl-CoA + taurine
-
487368
Rattus norvegicus
CoA + taurochenodeoxycholate
-
-
-
?
2.3.1.65
choloyl-CoA + beta-alanine
-
487368
Rattus norvegicus
CoA + beta-alanylcholate
-
-
-
?
2.3.1.65
choloyl-CoA + glycine
-
487368
Rattus norvegicus
CoA + glycocholate
-
487368
Rattus norvegicus
?
2.3.1.65
choloyl-CoA + taurine
preferred substrate
487368
Rattus norvegicus
CoA + taurocholate
-
487368
Rattus norvegicus
?
2.3.1.65
choloyl-CoA + taurine
taurine conjugates predominate in rat bile at normal hepatocellular concentrations of glycine and taurine
487368
Rattus norvegicus
CoA + taurocholate
-
-
-
?
2.3.1.65
deoxycholoyl-CoA + aminomethanesulfonate
-
487368
Rattus norvegicus
CoA + aminomethanesulfonyldeoxycholate
-
-
-
?
2.3.1.65
deoxycholoyl-CoA + glycine
-
487368
Rattus norvegicus
CoA + glycodeoxycholate
-
487368
Rattus norvegicus
?
2.3.1.65
deoxycholoyl-CoA + taurine
-
487368
Rattus norvegicus
CoA + taurodeoxycholate
-
487368
Rattus norvegicus
?
2.3.1.65
lithocholoyl-CoA + glycine
-
487368
Rattus norvegicus
CoA + glycolithocholate
-
-
-
?
2.3.1.65
lithocholoyl-CoA + taurine
-
487368
Rattus norvegicus
CoA + taurolithocholate
-
-
-
?
2.3.1.65
additional information
not: D-alanine, L-arginine, L-lysine, 2-hydroxyethanesulfonate, 2-aminoethanesulfonate, 1-aminoethanephosphonate and 2-aminoethanephosphonate, 3-aminopropanesulfonate
487368
Rattus norvegicus
?
-
-
-
-
2.3.1.65
additional information
not as acyl-CoA donors: acetyl-CoA, phenylacetyl-CoA and benzoyl-CoA
487368
Rattus norvegicus
?
-
-
-
-
2.3.1.65
additional information
not as acyl-CoA donors: succinoyl-CoA, palmitoyl-CoA
487368
Rattus norvegicus
?
-
-
-
-
2.3.1.65
additional information
not: L-alpha-alanine, L-glutamine, L-ornithine
487368
Rattus norvegicus
?
-
-
-
-
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.3.1.65
30
-
assay at
Rattus norvegicus
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.3.1.65
45
-
stable below
Rattus norvegicus
2.3.1.65
50
-
inactivation above
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.65
additional information
-
pI: 7.2
Rattus norvegicus
2.3.1.65
7.8
8
-
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.65
6
9
about 70% of maximal activity at pH 6.0, about half-maximal activity at pH 9.0, inactivation rate is similar for both taurine and glycine
Rattus norvegicus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.3.1.65
6
-
phosphate buffer, t1/2: 2 h
Rattus norvegicus
2.3.1.65
9
-
Tris-buffer, t1/2: 3 h
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.65
5,5'-dithiobis(2-nitrobenzoic acid)
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.65
additional information
-
additional information
-
Rattus norvegicus
2.3.1.65
0.8
-
aminomethanesulfonate
+ 0.05 mM deoxycholoyl-CoA
Rattus norvegicus
2.3.1.65
0.8
-
taurine
+ 0.05 mM deoxycholoyl-CoA
Rattus norvegicus
2.3.1.65
31
-
glycine
+ 0.05 mM deoxycholoyl-CoA
Rattus norvegicus
2.3.1.65
175
-
beta-Alanine
-
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.65
choloyl-CoA + taurine
Rattus norvegicus
taurine conjugates predominate in rat bile at normal hepatocellular concentrations of glycine and taurine
CoA + taurocholate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.65
partial, 200fold
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.65
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.3.1.65
additional information
-
-
Rattus norvegicus
2.3.1.65
1.99
-
taurine
Rattus norvegicus
2.3.1.65
3.88
-
glycine
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.65
-70C, several weeks, stable
Rattus norvegicus
2.3.1.65
0 to 20C, several h, stable
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.65
chenodeoxycholoyl-CoA + glycine
-
487368
Rattus norvegicus
CoA + glycochenodeoxycholate
-
-
-
?
2.3.1.65
chenodeoxycholoyl-CoA + taurine
-
487368
Rattus norvegicus
CoA + taurochenodeoxycholate
-
-
-
?
2.3.1.65
choloyl-CoA + beta-alanine
-
487368
Rattus norvegicus
CoA + beta-alanylcholate
-
-
-
?
2.3.1.65
choloyl-CoA + glycine
-
487368
Rattus norvegicus
CoA + glycocholate
-
487368
Rattus norvegicus
?
2.3.1.65
choloyl-CoA + taurine
preferred substrate
487368
Rattus norvegicus
CoA + taurocholate
-
487368
Rattus norvegicus
?
2.3.1.65
choloyl-CoA + taurine
taurine conjugates predominate in rat bile at normal hepatocellular concentrations of glycine and taurine
487368
Rattus norvegicus
CoA + taurocholate
-
-
-
?
2.3.1.65
deoxycholoyl-CoA + aminomethanesulfonate
-
487368
Rattus norvegicus
CoA + aminomethanesulfonyldeoxycholate
-
-
-
?
2.3.1.65
deoxycholoyl-CoA + glycine
-
487368
Rattus norvegicus
CoA + glycodeoxycholate
-
487368
Rattus norvegicus
?
2.3.1.65
deoxycholoyl-CoA + taurine
-
487368
Rattus norvegicus
CoA + taurodeoxycholate
-
487368
Rattus norvegicus
?
2.3.1.65
lithocholoyl-CoA + glycine
-
487368
Rattus norvegicus
CoA + glycolithocholate
-
-
-
?
2.3.1.65
lithocholoyl-CoA + taurine
-
487368
Rattus norvegicus
CoA + taurolithocholate
-
-
-
?
2.3.1.65
additional information
not: D-alanine, L-arginine, L-lysine, 2-hydroxyethanesulfonate, 2-aminoethanesulfonate, 1-aminoethanephosphonate and 2-aminoethanephosphonate, 3-aminopropanesulfonate
487368
Rattus norvegicus
?
-
-
-
-
2.3.1.65
additional information
not as acyl-CoA donors: acetyl-CoA, phenylacetyl-CoA and benzoyl-CoA
487368
Rattus norvegicus
?
-
-
-
-
2.3.1.65
additional information
not as acyl-CoA donors: succinoyl-CoA, palmitoyl-CoA
487368
Rattus norvegicus
?
-
-
-
-
2.3.1.65
additional information
not: L-alpha-alanine, L-glutamine, L-ornithine
487368
Rattus norvegicus
?
-
-
-
-
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.3.1.65
30
-
assay at
Rattus norvegicus
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.3.1.65
45
-
stable below
Rattus norvegicus
2.3.1.65
50
-
inactivation above
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.65
additional information
-
pI: 7.2
Rattus norvegicus
2.3.1.65
7.8
8
-
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.65
6
9
about 70% of maximal activity at pH 6.0, about half-maximal activity at pH 9.0, inactivation rate is similar for both taurine and glycine
Rattus norvegicus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.3.1.65
6
-
phosphate buffer, t1/2: 2 h
Rattus norvegicus
2.3.1.65
9
-
Tris-buffer, t1/2: 3 h
Rattus norvegicus