BRENDA - Enzyme Database

A new class of N-hydroxycinnamoyltransferases. Purification, cloning, and expression of barley agmatine coumaroyltransferase (EC 2.3.1.64)

Burhenne, K.; Kristensen, B.K.; Rasmussen, S.K.; J. Biol. Chem. 278, 13919-13927 (2003)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.64
1-mercapto-2,3-propanediol
1 mM is required for optimal activity
Hordeum vulgare
2.3.1.64
2-mercaptoethanol
1 mM is required for optimal activity
Hordeum vulgare
Cloned(Commentary)
EC Number
Commentary
Organism
2.3.1.64
expression in Escherichia coli
Hordeum vulgare
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.64
CuSO4
10 mM, 85% inhibition
Hordeum vulgare
2.3.1.64
dithiothreitol
30% inhibition
Hordeum vulgare
2.3.1.64
MnCl2
10 mM, 29% inhibition
Hordeum vulgare
2.3.1.64
ZnSO4
10 mM, 99% inhibition
Hordeum vulgare
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.64
0.001
-
4-Coumaroyl-CoA
isoenzyme 1
Hordeum vulgare
2.3.1.64
0.0016
-
4-Coumaroyl-CoA
isoenzyme 3
Hordeum vulgare
2.3.1.64
0.0017
-
feruloyl-CoA
isoenzyme 3
Hordeum vulgare
2.3.1.64
0.0021
-
4-Coumaroyl-CoA
isoenzyme 2; recombinant enzyme
Hordeum vulgare
2.3.1.64
0.0041
-
feruloyl-CoA
isoenzyme 2
Hordeum vulgare
2.3.1.64
0.0043
-
caffeoyl-CoA
isoenzyme 2; recombinant enzyme
Hordeum vulgare
2.3.1.64
0.0052
-
agmatine
isoenzyme 1; isoenzyme 3
Hordeum vulgare
2.3.1.64
0.0053
-
caffeoyl-CoA
isoenzyme 3
Hordeum vulgare
2.3.1.64
0.0058
-
caffeoyl-CoA
isoenzyme 1
Hordeum vulgare
2.3.1.64
0.0061
-
feruloyl-CoA
isoenzyme 1
Hordeum vulgare
2.3.1.64
0.0065
-
agmatine
isoenzyme 2
Hordeum vulgare
2.3.1.64
0.0066
-
feruloyl-CoA
recombinant enzyme
Hordeum vulgare
2.3.1.64
0.0081
-
agmatine
recombinant enzyme
Hordeum vulgare
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.64
40000
-
-
Hordeum vulgare
2.3.1.64
48000
-
1 * 48000, SDS-PAGE
Hordeum vulgare
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.64
Hordeum vulgare
A9ZPJ6
isoenzymes 1-3
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.64
Blue Sepharose, t-butyl-Sepharose, Resource Q, Superose 12 HR
Hordeum vulgare
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.64
1.78
-
-
Hordeum vulgare
2.3.1.64
11.34
-
recombinant enzyme
Hordeum vulgare
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.64
0°C, 100 mM Tris, pH 7.5, 1 mM EDTA, 10 mM 2-mercaptoethanol, 50 mM KCl
Hordeum vulgare
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.64
4-coumaroyl-CoA + agmatine
-
487363
Hordeum vulgare
CoA + 4-coumaroylagmatine
-
-
-
-
2.3.1.64
caffeoyl-CoA + agmatine
-
487363
Hordeum vulgare
CoA + caffeoylagmatine
-
-
-
-
2.3.1.64
feruloyl-CoA + agmatine
-
487363
Hordeum vulgare
CoA + feruloyl agmatine
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.64
monomer
1 * 48000, SDS-PAGE
Hordeum vulgare
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.64
40
-
-
Hordeum vulgare
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.3.1.64
20
50
-
Hordeum vulgare
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.64
7.5
-
-
Hordeum vulgare
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.64
6.9
8.1
half-maximal activity at pH 6.9 and pH 8.1
Hordeum vulgare
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.64
1-mercapto-2,3-propanediol
1 mM is required for optimal activity
Hordeum vulgare
2.3.1.64
2-mercaptoethanol
1 mM is required for optimal activity
Hordeum vulgare
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.64
expression in Escherichia coli
Hordeum vulgare
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.64
CuSO4
10 mM, 85% inhibition
Hordeum vulgare
2.3.1.64
dithiothreitol
30% inhibition
Hordeum vulgare
2.3.1.64
MnCl2
10 mM, 29% inhibition
Hordeum vulgare
2.3.1.64
ZnSO4
10 mM, 99% inhibition
Hordeum vulgare
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.64
0.001
-
4-Coumaroyl-CoA
isoenzyme 1
Hordeum vulgare
2.3.1.64
0.0016
-
4-Coumaroyl-CoA
isoenzyme 3
Hordeum vulgare
2.3.1.64
0.0017
-
feruloyl-CoA
isoenzyme 3
Hordeum vulgare
2.3.1.64
0.0021
-
4-Coumaroyl-CoA
isoenzyme 2; recombinant enzyme
Hordeum vulgare
2.3.1.64
0.0041
-
feruloyl-CoA
isoenzyme 2
Hordeum vulgare
2.3.1.64
0.0043
-
caffeoyl-CoA
isoenzyme 2; recombinant enzyme
Hordeum vulgare
2.3.1.64
0.0052
-
agmatine
isoenzyme 1; isoenzyme 3
Hordeum vulgare
2.3.1.64
0.0053
-
caffeoyl-CoA
isoenzyme 3
Hordeum vulgare
2.3.1.64
0.0058
-
caffeoyl-CoA
isoenzyme 1
Hordeum vulgare
2.3.1.64
0.0061
-
feruloyl-CoA
isoenzyme 1
Hordeum vulgare
2.3.1.64
0.0065
-
agmatine
isoenzyme 2
Hordeum vulgare
2.3.1.64
0.0066
-
feruloyl-CoA
recombinant enzyme
Hordeum vulgare
2.3.1.64
0.0081
-
agmatine
recombinant enzyme
Hordeum vulgare
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.64
40000
-
-
Hordeum vulgare
2.3.1.64
48000
-
1 * 48000, SDS-PAGE
Hordeum vulgare
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.64
Blue Sepharose, t-butyl-Sepharose, Resource Q, Superose 12 HR
Hordeum vulgare
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.64
1.78
-
-
Hordeum vulgare
2.3.1.64
11.34
-
recombinant enzyme
Hordeum vulgare
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.64
0°C, 100 mM Tris, pH 7.5, 1 mM EDTA, 10 mM 2-mercaptoethanol, 50 mM KCl
Hordeum vulgare
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.64
4-coumaroyl-CoA + agmatine
-
487363
Hordeum vulgare
CoA + 4-coumaroylagmatine
-
-
-
-
2.3.1.64
caffeoyl-CoA + agmatine
-
487363
Hordeum vulgare
CoA + caffeoylagmatine
-
-
-
-
2.3.1.64
feruloyl-CoA + agmatine
-
487363
Hordeum vulgare
CoA + feruloyl agmatine
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.64
monomer
1 * 48000, SDS-PAGE
Hordeum vulgare
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.64
40
-
-
Hordeum vulgare
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.3.1.64
20
50
-
Hordeum vulgare
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.64
7.5
-
-
Hordeum vulgare
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.64
6.9
8.1
half-maximal activity at pH 6.9 and pH 8.1
Hordeum vulgare