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Literature summary extracted from

  • Burhenne, K.; Kristensen, B.K.; Rasmussen, S.K.
    A new class of N-hydroxycinnamoyltransferases. Purification, cloning, and expression of barley agmatine coumaroyltransferase (EC 2.3.1.64) (2003), J. Biol. Chem., 278, 13919-13927.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.64 1-mercapto-2,3-propanediol 1 mM is required for optimal activity Hordeum vulgare
2.3.1.64 2-mercaptoethanol 1 mM is required for optimal activity Hordeum vulgare

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.64 expression in Escherichia coli Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.64 CuSO4 10 mM, 85% inhibition Hordeum vulgare
2.3.1.64 dithiothreitol 30% inhibition Hordeum vulgare
2.3.1.64 MnCl2 10 mM, 29% inhibition Hordeum vulgare
2.3.1.64 ZnSO4 10 mM, 99% inhibition Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.64 0.001
-
 4-coumaroyl-CoA isoenzyme 1 Hordeum vulgare
2.3.1.64 0.0016
-
 4-coumaroyl-CoA isoenzyme 3 Hordeum vulgare
2.3.1.64 0.0017
-
 feruloyl-CoA isoenzyme 3 Hordeum vulgare
2.3.1.64 0.0021
-
 4-coumaroyl-CoA isoenzyme 2 Hordeum vulgare
2.3.1.64 0.0021
-
 4-coumaroyl-CoA recombinant enzyme Hordeum vulgare
2.3.1.64 0.0041
-
 feruloyl-CoA isoenzyme 2 Hordeum vulgare
2.3.1.64 0.0043
-
 caffeoyl-CoA isoenzyme 2 Hordeum vulgare
2.3.1.64 0.0043
-
 caffeoyl-CoA recombinant enzyme Hordeum vulgare
2.3.1.64 0.0052
-
 agmatine isoenzyme 1 Hordeum vulgare
2.3.1.64 0.0052
-
 agmatine isoenzyme 3 Hordeum vulgare
2.3.1.64 0.0053
-
 caffeoyl-CoA isoenzyme 3 Hordeum vulgare
2.3.1.64 0.0058
-
 caffeoyl-CoA isoenzyme 1 Hordeum vulgare
2.3.1.64 0.0061
-
 feruloyl-CoA isoenzyme 1 Hordeum vulgare
2.3.1.64 0.0065
-
 agmatine isoenzyme 2 Hordeum vulgare
2.3.1.64 0.0066
-
 feruloyl-CoA recombinant enzyme Hordeum vulgare
2.3.1.64 0.0081
-
 agmatine recombinant enzyme Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.64 40000
-
-
 Hordeum vulgare
2.3.1.64 48000
-
 1 * 48000, SDS-PAGE Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.64 Hordeum vulgare A9ZPJ6 isoenzymes 1-3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.64 Blue Sepharose, t-butyl-Sepharose, Resource Q, Superose 12 HR Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.64 1.78
-
-
 Hordeum vulgare
2.3.1.64 11.34
-
 recombinant enzyme Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
2.3.1.64 0°C, 100 mM Tris, pH 7.5, 1 mM EDTA, 10 mM 2-mercaptoethanol, 50 mM KCl Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.64 4-coumaroyl-CoA + agmatine
-
 Hordeum vulgare CoA + 4-coumaroylagmatine
-
 ?
2.3.1.64 caffeoyl-CoA + agmatine
-
 Hordeum vulgare CoA + caffeoylagmatine
-
 ?
2.3.1.64 feruloyl-CoA + agmatine
-
 Hordeum vulgare CoA + feruloyl agmatine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.64 monomer 1 * 48000, SDS-PAGE Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.64 40
-
-
 Hordeum vulgare

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.64 20 50
-
 Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.64 7.5
-
-
 Hordeum vulgare

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.64 6.9 8.1 half-maximal activity at pH 6.9 and pH 8.1 Hordeum vulgare