BRENDA - Enzyme Database

Agmatine coumaroyltransferase (barley seedlings)

Bird, C.R.; Smith, T.A.; Methods Enzymol. 94, 344-347 (1983)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.64
0.00074
-
4-Coumaroyl-CoA
-
Hordeum vulgare
2.3.1.64
0.012
-
agmatine
-
Hordeum vulgare
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.64
40000
-
gel filtration
Hordeum vulgare
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.64
Hordeum vulgare
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.64
ammonium sulfate, Biogel A, Sepharose 4B-agmatine
Hordeum vulgare
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.64
seedling
maximal activity 3-4 days after germination at 22°C in the dark; shoots, not seeds
Hordeum vulgare
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.64
0.393
-
-
Hordeum vulgare
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.64
-40°C, concentrated preparation at least 1 month, negligible loss of activity
Hordeum vulgare
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.64
4-coumaroyl-CoA + agmatine
no activity with putrescine, spermidine, spermine, cadaverine, arginine, homoarginine
487361
Hordeum vulgare
CoA + 4-coumaroylagmatine
-
487361
Hordeum vulgare
?
2.3.1.64
caffeoyl-CoA + agmatine
-
487361
Hordeum vulgare
CoA + caffeoylagmatine
-
487361
Hordeum vulgare
?
2.3.1.64
feruloyl-CoA + agmatine
-
487361
Hordeum vulgare
CoA + feruloyl agmatine
-
487361
Hordeum vulgare
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.64
7.5
-
-
Hordeum vulgare
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.64
7
8
approx. half-maximal activity at pH 7.0 and 8.0
Hordeum vulgare
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.64
0.00074
-
4-Coumaroyl-CoA
-
Hordeum vulgare
2.3.1.64
0.012
-
agmatine
-
Hordeum vulgare
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.64
40000
-
gel filtration
Hordeum vulgare
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.64
ammonium sulfate, Biogel A, Sepharose 4B-agmatine
Hordeum vulgare
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.64
seedling
maximal activity 3-4 days after germination at 22°C in the dark; shoots, not seeds
Hordeum vulgare
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.64
0.393
-
-
Hordeum vulgare
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.64
-40°C, concentrated preparation at least 1 month, negligible loss of activity
Hordeum vulgare
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.64
4-coumaroyl-CoA + agmatine
no activity with putrescine, spermidine, spermine, cadaverine, arginine, homoarginine
487361
Hordeum vulgare
CoA + 4-coumaroylagmatine
-
487361
Hordeum vulgare
?
2.3.1.64
caffeoyl-CoA + agmatine
-
487361
Hordeum vulgare
CoA + caffeoylagmatine
-
487361
Hordeum vulgare
?
2.3.1.64
feruloyl-CoA + agmatine
-
487361
Hordeum vulgare
CoA + feruloyl agmatine
-
487361
Hordeum vulgare
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.64
7.5
-
-
Hordeum vulgare
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.64
7
8
approx. half-maximal activity at pH 7.0 and 8.0
Hordeum vulgare