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Literature summary extracted from

  • Parry, L.; Lopez-Ballester, J.; Wiest, L.; Pegg, A.E.
    Effect of expression of human spermidine/spermine N1-acetyltransferase in Escherichia coli (1995), Biochemistry, 34, 2701-2709.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.57 additional information N1,N12-bis(ethyl)spermine is a potent inducer of enzyme in human tumor cells Homo sapiens
2.3.1.57 additional information enzyme is induced by isopropyl beta-D-thiogalactopyranoside Homo sapiens

Application

EC Number Application Comment Organism
2.3.1.57 medicine induction of enzyme may be critical for the inhibition of tumor cell growth Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.57 cloning and expression of cDNA encoding enzyme in Escherichia coli DH5alpha leads to a significant reduction in the cell growth rate Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.57 0.012
-
 15-deoxyspergualin
-
 Homo sapiens
2.3.1.57 0.025
-
 spermine
-
 Homo sapiens
2.3.1.57 0.06
-
 spermidine
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.57 soluble recombinant enzyme, expressed in Escherichia coli DH5alpha, is present at a level of about 2% of the soluble protein Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.57 21000
-
 x * 21000 + x * 23000, two bands, enzyme is probably a tetramer, recombinant enzyme, SDS-PAGE Homo sapiens
2.3.1.57 23000
-
 x * 21000 + x * 23000, two bands, enzyme is probably a tetramer, recombinant enzyme, SDS-PAGE Homo sapiens
2.3.1.57 75000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.57 additional information Homo sapiens acetylation is a physiological response to convert excess polyamines to a physiologically inert form which is readily excreted ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.57 Homo sapiens
-
 recombinant enzyme, expressed in Escherichia coli DH5alpha
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.57 no modification no posttranslational modification specific to eukaryotes is needed for enzyme activity Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.57 62fold purification of recombinant enzyme, expressed in Escherichia coli DH5alpha Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.57 additional information
-
-
 Homo sapiens
2.3.1.57 45
-
 spermidine Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.3.1.57 -70°C, several weeks, stable Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.57 acetyl-CoA + (S)-2-[(3-aminopropyl)amino]ethylphosphoric acid WR-2721, about 10% of the rate with spermidine Homo sapiens CoA + N-acetyl-(S)-2-[(3-aminopropyl)amino]ethylphosphoric acid
-
 ?
2.3.1.57 acetyl-CoA + (S)-2-[(3-aminopropyl)amino]propylphosphoric acid WR-44923, about 10% of the rate with spermidine Homo sapiens CoA + N-acetyl-(S)-2-[(3-aminopropyl)amino]propylphosphoric acid
-
 ?
2.3.1.57 acetyl-CoA + 15-deoxyspergualin antitumor and immunosuppressive agent 15-deoxyspergualin, about 18% of the rate with spermidine Homo sapiens ?
-
 ?
2.3.1.57 acetyl-CoA + 2-[(aminopropyl)amino]ethanethiol radioprotective drug WR-1065, lower affinity than for spermidine, about 10% of the rate with spermidine Homo sapiens CoA + N-acetyl-2-[(aminopropyl)amino]ethanethiol
-
 ?
2.3.1.57 acetyl-CoA + N-(n-butyl)-1,3-diaminopropane weak substrate, lower affinity than for spermidine, 1.3% of the rate with spermidine Homo sapiens CoA + N1-acetyl-N3-(n-butyl)-1,3-diaminopropane
-
 ?
2.3.1.57 acetyl-CoA + spermidine
-
 Homo sapiens CoA + N1-acetylspermidine product is exclusively N1-acetylspermidine ?
2.3.1.57 acetyl-CoA + spermine about 40% of the rate with spermidine Homo sapiens CoA + N1-acetylspermine
-
 ?
2.3.1.57 additional information not: 1-methylspermidine, WR-2822 Homo sapiens ?
-
 ?
2.3.1.57 additional information enzyme requires a substrate with the structure H2N(CH2)3NHR and acetylates the primary amino group Homo sapiens ?
-
 ?
2.3.1.57 additional information not: putrescine Homo sapiens ?
-
 ?
2.3.1.57 additional information acetylation is a physiological response to convert excess polyamines to a physiologically inert form which is readily excreted Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.57 tetramer x * 21000 + x * 23000, two bands, enzyme is probably a tetramer, recombinant enzyme, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.57 30
-
 assay at Homo sapiens