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Literature summary extracted from

  • Della Ragione, F.; Pegg, A.E.
    Spermidine N1-acetyltransferase (1983), Methods Enzymol., 94, 321-325.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.57 additional information enzyme is induced by treatment with hepatotoxins, e.g. carbon tetrachloride Rattus norvegicus
2.3.1.57 additional information enzyme is induced by growth hormone, thioacetamide, dialkylnitrosamines, folic acid and spermidine Rattus norvegicus

General Stability

EC Number General Stability Organism
2.3.1.57 sensitive to incubation in dilute solutions, bovine serum albumin, 0.5 mg/ml, stabilizes Rattus norvegicus
2.3.1.57 unstable enzyme, spermidine stabilizes Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.57 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.57 60000
-
 2 * 60000, SDS-PAGE Rattus norvegicus
2.3.1.57 114000
-
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.57 Rattus norvegicus
-
 female Sprague-Dawley rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.57 112000fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.57 liver carbon tetrachloride induced liver Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.57 8.102
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.3.1.57 -20°C, 50 mM Tris-HCl buffer, pH 7.5, 10 mM spermidine, per week, 30% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.57 acetyl-CoA + 1,3-diaminopropane at a low rate Rattus norvegicus CoA + N1-acetyl-1,3-diaminopropane
-
 ?
2.3.1.57 acetyl-CoA + N1-acetylspermine
-
 Rattus norvegicus CoA + N1,N12-diacetylspermine
-
 ?
2.3.1.57 acetyl-CoA + spermidine
-
 Rattus norvegicus CoA + N1-acetylspermidine product is exclusively N1-acetylspermidine ?
2.3.1.57 acetyl-CoA + spermine
-
 Rattus norvegicus CoA + N1-acetylspermine
-
 ?
2.3.1.57 acetyl-CoA + sym-norspermidine
-
 Rattus norvegicus CoA + N1-acetyl-sym-norspermidine
-
 ?
2.3.1.57 acetyl-CoA + sym-norspermine
-
 Rattus norvegicus CoA + N1-acetyl-sym-norspermine
-
 ?
2.3.1.57 additional information not: histone Rattus norvegicus ?
-
 ?
2.3.1.57 additional information enzyme requires a substrate with the structure H2N(CH2)3NHR and acetylates the primary amino group Rattus norvegicus ?
-
 ?
2.3.1.57 additional information not: putrescine Rattus norvegicus ?
-
 ?
2.3.1.57 additional information not: sym-homospermidine Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.57 dimer 2 * 60000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.57 30
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.57 additional information
-
 very sensitive to heat denaturation Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.57 additional information no known cofactors Rattus norvegicus