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Literature summary extracted from

  • Lehtioe, L.; Leppaenen, V.M.; Kozarich, J.W.; Goldman, A.
    Structure of Escherichia coli pyruvate formate-lyase with pyruvate (2002), Acta Crystallogr. Sect. D, 58, 2209-2212.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.54 oxamate obligatory component of the activation reaction Escherichia coli
2.3.1.54 pyruvate obligatory component of the activation reaction Escherichia coli
2.3.1.54 S-adenosylmethionine obligatory component of the activation reaction Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.54 recombinant PFL expressed in Escherichia coli BL21 (DE3) Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.54 space group P4(3)2(1)2, cell parameters a = b = 158.36 A, c = 159.30 A Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.54 85000
-
 2 * 85000, homodimer, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.54 pyruvate + CoA Escherichia coli
-
 acetyl-CoA + formate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.54 Escherichia coli P09373
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.54
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.54 pyruvate + CoA
-
 Escherichia coli acetyl-CoA + formate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.54 dimer 2 * 85000, homodimer, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.3.1.54 PFL
-
 Escherichia coli