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Literature summary extracted from

  • Knappe, J.; Blaschkowski, H.P.
    Pyruvate formate-lyase from Escherichia coli and its activation system (1975), Methods Enzymol., 41B, 508-518.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.54 oxamate obligatory component of the activation reaction Escherichia coli
2.3.1.54 pyruvate obligatory component of the activation reaction Escherichia coli
2.3.1.54 S-adenosylmethionine obligatory component of the activation reaction Escherichia coli

General Stability

EC Number General Stability Organism
2.3.1.54 virtually stable at 0°C and pH 8.0 if kept in media which display a redoxpotential of 0.2 V or below Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.54 S-adenosyl-L-homocysteine
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.54 0.0068
-
 CoA
-
 Escherichia coli
2.3.1.54 0.051
-
 acetyl-CoA
-
 Escherichia coli
2.3.1.54 2
-
 pyruvate
-
 Escherichia coli
2.3.1.54 24
-
 formate
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.54 70000
-
 2 * 70000 Escherichia coli
2.3.1.54 140000
-
 gel filtration, sedimentation velocity Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.54 pyruvate + CoA Enterococcus faecalis
-
 acetyl-CoA + formate
-
 r
2.3.1.54 pyruvate + CoA Clostridium sp.
-
 acetyl-CoA + formate
-
 r
2.3.1.54 pyruvate + CoA Escherichia coli central reaction in the anaerobic metabolism acetyl-CoA + formate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.54 Clostridium sp.
-
-
-
2.3.1.54 Enterococcus faecalis
-
 previously Streptococcus faecalis
-
2.3.1.54 Escherichia coli
-
 K12
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.3.1.54 extreme sensitivity towards inactivation by oxygen, admittance of oxygen inactivates completely within a few seconds Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.54 partially Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.54 25
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.3.1.54 -20°C, stable for at least several months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.54 acetyl-CoA + formate
-
 Escherichia coli pyruvate + CoA
-
 r
2.3.1.54 pyruvate + CoA
-
 Escherichia coli acetyl-CoA + formate
-
 r
2.3.1.54 pyruvate + CoA
-
 Enterococcus faecalis acetyl-CoA + formate
-
 r
2.3.1.54 pyruvate + CoA
-
 Clostridium sp. acetyl-CoA + formate
-
 r
2.3.1.54 pyruvate + CoA central reaction in the anaerobic metabolism Escherichia coli acetyl-CoA + formate
-
 r
2.3.1.54 pyruvate + formate carboxyl group exchange reaction Escherichia coli formate + pyruvate
-
 r
2.3.1.54 pyruvate + formate carboxyl group exchange reaction Enterococcus faecalis formate + pyruvate
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.54 dimer 2 * 70000 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.3.1.54 PFL
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.54 30
-
 activity declines with a half-time of 50 min Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.54 7.8 8.4
-
 Escherichia coli