Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Knappe, J.; Balschkowski, H.P.; Gröbner, P.; Schmitt, T.
    Pyruvate formate-lyase of Escherichia coli: the acetyl-enzyme intermediate (1974), Eur. J. Biochem., 50, 253-263.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.54 formate product inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.54 0.0068
-
 CoA
-
 Escherichia coli
2.3.1.54 0.051
-
 acetyl-CoA
-
 Escherichia coli
2.3.1.54 2.05
-
 pyruvate
-
 Escherichia coli
2.3.1.54 24
-
 formate
-
 Escherichia coli
2.3.1.54 24.5
-
 formate
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.54 150000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.54 acetyl-CoA + formate Escherichia coli
-
 pyruvate + CoA
-
 r
2.3.1.54 pyruvate + CoA Escherichia coli key role in the metabolism acetyl-CoA + formate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.54 Escherichia coli
-
 K12
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.3.1.54 extreme sensitivity towards inactivation by oxygen, admittance of oxygen inactivates completely within a few seconds Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.54
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.54 60
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.54 acetyl-CoA + formate
-
 Escherichia coli pyruvate + CoA
-
 r
2.3.1.54 pyruvate + CoA
-
 Escherichia coli acetyl-CoA + formate
-
 r
2.3.1.54 pyruvate + CoA key role in the metabolism Escherichia coli acetyl-CoA + formate
-
 r
2.3.1.54 pyruvate + dithiothreitol
-
 Escherichia coli S-acetyl-dithiothreitol + formate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.54 phosphotransferase system enzyme I
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.54 3.8
-
 formate
-
 Escherichia coli
2.3.1.54 11
-
 pyruvate
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.54 7.8 8.5 forward direction Escherichia coli