BRENDA - Enzyme Database

ATP-independent fatty acyl-coenzyme A synthesis from phospholipid: coenzyme A-dependent transacylation activity toward lysophosphatidic acid catalyzed by acyl-coenzyme A:lysophosphatidic acid acyltransferase

Yamashita, A.; Kawagishi, N.; Miyashita, T.; Nagatsuka, T.; Sugiura, T.; Kume, K.; Shimizu, T.; Waku, K.; J. Biol. Chem. 276, 26745-26752 (2001)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.51
isopropyl-beta-D-thiogalactopyranoside
induction of the enzyme gene
Mus musculus
Cloned(Commentary)
EC Number
Commentary
Organism
2.3.1.51
expression in JC201 Escherichia coli cells
Mus musculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.51
32000
-
Western blotting
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.51
Mus musculus
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
recombinant enzyme can catalyse ATP-independent acyl-CoA synthetic activity and CoA-dependent transacylation activity. Acyl-CoA synthetase is not involved in the process
487210
Mus musculus
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
r
2.3.1.51
acyl-CoA + 1-O-alkyl-sn-glycerol 3-phosphate
the activity is approximately half of that toward 1-acyl-sn-glycerol 3-phosphate
487210
Mus musculus
CoA + ?
-
487210
Mus musculus
?
2.3.1.51
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
r
2.3.1.51
arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
?
2.3.1.51
linoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
CoA + 1-acyl-2-linoleoyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
?
2.3.1.51
linoleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
CoA + 1-oleoyl-2-linoleoyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
r
2.3.1.51
additional information
lysophospholipids such as 1-acyl-sn-glycero-3-phosphocholine, 1-acyl-sn-glycero-3-phosphoethanolamine, 1-acyl-sn-glycero-3-phosphoinositol and lysoplatelet-activating factor do not serve as acyl acceptor substrates. Free fatty acids do not serve as acyl donors. The enzyme shows a broad specificity for acyl-CoAs
487210
Mus musculus
?
-
-
-
-
2.3.1.51
additional information
reverse reaction of the enzyme: desulfo-CoA and dephospho-COA do not serve as substrates for acyl-CoA synthesis
487210
Mus musculus
?
-
-
-
-
2.3.1.51
myristoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
CoA + 1-acyl-2-myristoyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
?
2.3.1.51
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
?
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
?
2.3.1.51
stearoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
CoA + 1-acyl-2-stearoyl-sn-glycerol 3-phosphate
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.51
30
-
assay at
Mus musculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.51
7.4
-
assay at
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.51
isopropyl-beta-D-thiogalactopyranoside
induction of the enzyme gene
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.51
expression in JC201 Escherichia coli cells
Mus musculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.51
32000
-
Western blotting
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
recombinant enzyme can catalyse ATP-independent acyl-CoA synthetic activity and CoA-dependent transacylation activity. Acyl-CoA synthetase is not involved in the process
487210
Mus musculus
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
r
2.3.1.51
acyl-CoA + 1-O-alkyl-sn-glycerol 3-phosphate
the activity is approximately half of that toward 1-acyl-sn-glycerol 3-phosphate
487210
Mus musculus
CoA + ?
-
487210
Mus musculus
?
2.3.1.51
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
r
2.3.1.51
arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
?
2.3.1.51
linoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
CoA + 1-acyl-2-linoleoyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
?
2.3.1.51
linoleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
CoA + 1-oleoyl-2-linoleoyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
r
2.3.1.51
additional information
lysophospholipids such as 1-acyl-sn-glycero-3-phosphocholine, 1-acyl-sn-glycero-3-phosphoethanolamine, 1-acyl-sn-glycero-3-phosphoinositol and lysoplatelet-activating factor do not serve as acyl acceptor substrates. Free fatty acids do not serve as acyl donors. The enzyme shows a broad specificity for acyl-CoAs
487210
Mus musculus
?
-
-
-
-
2.3.1.51
additional information
reverse reaction of the enzyme: desulfo-CoA and dephospho-COA do not serve as substrates for acyl-CoA synthesis
487210
Mus musculus
?
-
-
-
-
2.3.1.51
myristoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
CoA + 1-acyl-2-myristoyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
?
2.3.1.51
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
?
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
?
2.3.1.51
stearoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487210
Mus musculus
CoA + 1-acyl-2-stearoyl-sn-glycerol 3-phosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.51
30
-
assay at
Mus musculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.51
7.4
-
assay at
Mus musculus