BRENDA - Enzyme Database

Lysophosphatidic acid acyltransferase from immature coconut endosperm having medium chain length substrate specificity

Davies, H.M.; Hawkins, D.J.; Nelsen, J.S.; Phytochemistry 39, 989-996 (1995)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.51
additional information
stimulation of the enzyme by incorporation of the enzyme into the artificial membrane vesicles or by stripping of CHAPS from the enzyme into mixed detergent-phospholipid micelles. There is a critical order in which assay components had to be combined to obtain maximum stimulation
Cocos nucifera
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.51
2-mercaptoethanol
above 5 mM
Cocos nucifera
2.3.1.51
Ca2+
50% inhibition above 10 mM
Cocos nucifera
2.3.1.51
CHAPS
above 0.1%
Cocos nucifera
2.3.1.51
MgCl2
50% inhibition above 10 mM
Cocos nucifera
2.3.1.51
Mn2+
50% inhibition above 10 mM
Cocos nucifera
2.3.1.51
NaCl
above 200 mM
Cocos nucifera
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.51
membrane
-
Cocos nucifera
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.51
44000
-
estimated, high-speed supernatant, gel filtration
Cocos nucifera
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
Cocos nucifera
-
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
Cocos nucifera
?
2.3.1.51
additional information
Cocos nucifera
the enzyme has medium chain length substrate specificity appropriate to the biosynthesis of coconut oil
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.51
Cocos nucifera
-
coconut
-
Renatured (Commentary)
EC Number
Commentary
Organism
2.3.1.51
reassociation of the solubilized enzyme with soybean phospholipids
Cocos nucifera
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.51
endosperm
immature
Cocos nucifera
-
2.3.1.51
seed
-
Cocos nucifera
-
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.51
4C, high-speed supernatant preparation, 50% loss of activity, overnight
Cocos nucifera
2.3.1.51
4C, high-speed supernatant, reactivated with phospholipids, no loss of activity
Cocos nucifera
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487198
Cocos nucifera
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
487198
Cocos nucifera
?
2.3.1.51
additional information
acyl-CoAs containing 10:0, 12:0 and 14:0 acyl groups are the preferred acyl-donor substrates, acyl-ACPs are not utilized. There is a slight preference for 12:0-lysophosphatidic acid over 18:1-lysophosphatidic acid as acceptor substrate
487198
Cocos nucifera
?
-
-
-
-
2.3.1.51
additional information
the enzyme has medium chain length substrate specificity appropriate to the biosynthesis of coconut oil
487198
Cocos nucifera
?
-
-
-
-
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.3.1.51
30
-
assay at
Cocos nucifera
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.51
7.5
-
assay at
Cocos nucifera
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.3.1.51
6.5
7.5
lowering the assay pH from 7.5 to 6.5 results in a loss of approximately 20% of the enzyme activity
Cocos nucifera
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.51
additional information
stimulation of the enzyme by incorporation of the enzyme into the artificial membrane vesicles or by stripping of CHAPS from the enzyme into mixed detergent-phospholipid micelles. There is a critical order in which assay components had to be combined to obtain maximum stimulation
Cocos nucifera
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.51
2-mercaptoethanol
above 5 mM
Cocos nucifera
2.3.1.51
Ca2+
50% inhibition above 10 mM
Cocos nucifera
2.3.1.51
CHAPS
above 0.1%
Cocos nucifera
2.3.1.51
MgCl2
50% inhibition above 10 mM
Cocos nucifera
2.3.1.51
Mn2+
50% inhibition above 10 mM
Cocos nucifera
2.3.1.51
NaCl
above 200 mM
Cocos nucifera
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.51
membrane
-
Cocos nucifera
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.51
44000
-
estimated, high-speed supernatant, gel filtration
Cocos nucifera
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
Cocos nucifera
-
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
Cocos nucifera
?
2.3.1.51
additional information
Cocos nucifera
the enzyme has medium chain length substrate specificity appropriate to the biosynthesis of coconut oil
?
-
-
-
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.51
reassociation of the solubilized enzyme with soybean phospholipids
Cocos nucifera
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.51
endosperm
immature
Cocos nucifera
-
2.3.1.51
seed
-
Cocos nucifera
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.51
4C, high-speed supernatant preparation, 50% loss of activity, overnight
Cocos nucifera
2.3.1.51
4C, high-speed supernatant, reactivated with phospholipids, no loss of activity
Cocos nucifera
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487198
Cocos nucifera
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
487198
Cocos nucifera
?
2.3.1.51
additional information
acyl-CoAs containing 10:0, 12:0 and 14:0 acyl groups are the preferred acyl-donor substrates, acyl-ACPs are not utilized. There is a slight preference for 12:0-lysophosphatidic acid over 18:1-lysophosphatidic acid as acceptor substrate
487198
Cocos nucifera
?
-
-
-
-
2.3.1.51
additional information
the enzyme has medium chain length substrate specificity appropriate to the biosynthesis of coconut oil
487198
Cocos nucifera
?
-
-
-
-
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.3.1.51
30
-
assay at
Cocos nucifera
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.51
7.5
-
assay at
Cocos nucifera
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.3.1.51
6.5
7.5
lowering the assay pH from 7.5 to 6.5 results in a loss of approximately 20% of the enzyme activity
Cocos nucifera