BRENDA - Enzyme Database

The specificity of 1-acyl-sn-glycerol 3-phosphate acyltransferase in microsomal fractions from lactating cow mammary gland towards short, medium and long chain acyl-CoA esters

Marshall, M.O.; Knudsen, J.; Biochim. Biophys. Acta 489, 236-241 (1977)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.51
additional information
the enzyme can utilize the monomer form of long-chain acyl-CoA but it is inhibited by the micellar form of these substrates
Bos taurus
2.3.1.51
stearoyl-CoA
strongly inhibitory at 0.002 mM
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.51
microsome
-
Bos taurus
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.51
additional information
Bos taurus
1-palmitoyl- and 1-oleoyl-sn-glycerol 3-phosphate are probably the substrates for the enzyme in vivo, since palmitic and oleic acids are the major fatty acid components at the sn-1 position of cow milk triacylglycerols. The specificity of the enzyme plays a major role in determining the fatty acid composition at the sn-2 position of cow milk triacylglycerols, though the influence of other factors such as the in vivo concentration of the acyl-CoAs and the specificities of the monoacylglycerol acyltransferases cannot be ruled out
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.51
Bos taurus
-
Jersey cow
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.51
mammary gland
lactating
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
the enzyme is active towards acyl-CoAs of chain length C-8 to C-18
487193
Bos taurus
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
487193
Bos taurus
?
2.3.1.51
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
487193
Bos taurus
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
-
487193
Bos taurus
?
2.3.1.51
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
487193
Bos taurus
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
-
487193
Bos taurus
?
2.3.1.51
additional information
no acyl-donors are butyryl-CoA and hexanoyl-CoA
487193
Bos taurus
?
-
-
-
-
2.3.1.51
additional information
the chain length specificity of the enzyme in decreasing order is as follows: C16, C14, C12, C10, C8
487193
Bos taurus
?
-
-
-
-
2.3.1.51
additional information
1-palmitoyl- and 1-oleoyl-sn-glycerol 3-phosphate are probably the substrates for the enzyme in vivo, since palmitic and oleic acids are the major fatty acid components at the sn-1 position of cow milk triacylglycerols. The specificity of the enzyme plays a major role in determining the fatty acid composition at the sn-2 position of cow milk triacylglycerols, though the influence of other factors such as the in vivo concentration of the acyl-CoAs and the specificities of the monoacylglycerol acyltransferases cannot be ruled out
487193
Bos taurus
?
-
-
-
-
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
maximum activity is obtained with palmitoyl-CoA at a concentration of 0.002-0.003 mM
487193
Bos taurus
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
487193
Bos taurus
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.51
30
-
assay at
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.51
7.4
-
assay at
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.51
additional information
the enzyme can utilize the monomer form of long-chain acyl-CoA but it is inhibited by the micellar form of these substrates
Bos taurus
2.3.1.51
stearoyl-CoA
strongly inhibitory at 0.002 mM
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.51
microsome
-
Bos taurus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.51
additional information
Bos taurus
1-palmitoyl- and 1-oleoyl-sn-glycerol 3-phosphate are probably the substrates for the enzyme in vivo, since palmitic and oleic acids are the major fatty acid components at the sn-1 position of cow milk triacylglycerols. The specificity of the enzyme plays a major role in determining the fatty acid composition at the sn-2 position of cow milk triacylglycerols, though the influence of other factors such as the in vivo concentration of the acyl-CoAs and the specificities of the monoacylglycerol acyltransferases cannot be ruled out
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.51
mammary gland
lactating
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
the enzyme is active towards acyl-CoAs of chain length C-8 to C-18
487193
Bos taurus
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
487193
Bos taurus
?
2.3.1.51
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
487193
Bos taurus
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
-
487193
Bos taurus
?
2.3.1.51
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
487193
Bos taurus
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
-
487193
Bos taurus
?
2.3.1.51
additional information
no acyl-donors are butyryl-CoA and hexanoyl-CoA
487193
Bos taurus
?
-
-
-
-
2.3.1.51
additional information
the chain length specificity of the enzyme in decreasing order is as follows: C16, C14, C12, C10, C8
487193
Bos taurus
?
-
-
-
-
2.3.1.51
additional information
1-palmitoyl- and 1-oleoyl-sn-glycerol 3-phosphate are probably the substrates for the enzyme in vivo, since palmitic and oleic acids are the major fatty acid components at the sn-1 position of cow milk triacylglycerols. The specificity of the enzyme plays a major role in determining the fatty acid composition at the sn-2 position of cow milk triacylglycerols, though the influence of other factors such as the in vivo concentration of the acyl-CoAs and the specificities of the monoacylglycerol acyltransferases cannot be ruled out
487193
Bos taurus
?
-
-
-
-
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
maximum activity is obtained with palmitoyl-CoA at a concentration of 0.002-0.003 mM
487193
Bos taurus
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
487193
Bos taurus
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.51
30
-
assay at
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.51
7.4
-
assay at
Bos taurus