BRENDA - Enzyme Database

Properties of the microsomal acyl-CoA:sn-1-acyl-glycerol-3-phosphate acyltransferase from spinach (Spinacia oleracea L.) leaves

Hares, W.; Frentzen, M.; J. Plant Physiol. 131, 49-59 (1987)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.51
2-[3,5-di-(t-butyl-4-hydroxyphenyl)thio]hexanate
i.e. DH990, weak
Spinacia oleracea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.51
0.0014
-
palmitoyl-CoA
-
Spinacia oleracea
2.3.1.51
0.0028
-
oleoyl-CoA
-
Spinacia oleracea
2.3.1.51
0.003
-
1-palmitoyl-sn-glycerol 3-phosphate
with oleoyl-CoA
Spinacia oleracea
2.3.1.51
0.0053
-
1-oleoyl-sn-glycerol 3-phosphate
with oleoyl-CoA
Spinacia oleracea
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.51
endoplasmic reticulum
-
Spinacia oleracea
5783
-
2.3.1.51
microsome
-
Spinacia oleracea
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.51
Spinacia oleracea
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.51
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.3.1.51
0.0051
-
-
Spinacia oleracea
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.51
-20░C, microsomal preparation, at least 3 weeks
Spinacia oleracea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
487188
Spinacia oleracea
?
2.3.1.51
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
?
2.3.1.51
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
?
2.3.1.51
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
CoA + ?
-
487188
Spinacia oleracea
?
2.3.1.51
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
CoA + ?
-
487188
Spinacia oleracea
?
2.3.1.51
additional information
the enzyme uses preferentially those acyl donors and acceptors, which contain oleate, specificity and selectivity experiments
487188
Spinacia oleracea
?
-
-
-
-
2.3.1.51
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
?
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
?
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
2.3.1.51
25
-
assay at
Spinacia oleracea
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.51
9
11
broad, assay at pH 10
Spinacia oleracea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.51
2-[3,5-di-(t-butyl-4-hydroxyphenyl)thio]hexanate
i.e. DH990, weak
Spinacia oleracea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.51
0.0014
-
palmitoyl-CoA
-
Spinacia oleracea
2.3.1.51
0.0028
-
oleoyl-CoA
-
Spinacia oleracea
2.3.1.51
0.003
-
1-palmitoyl-sn-glycerol 3-phosphate
with oleoyl-CoA
Spinacia oleracea
2.3.1.51
0.0053
-
1-oleoyl-sn-glycerol 3-phosphate
with oleoyl-CoA
Spinacia oleracea
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.51
endoplasmic reticulum
-
Spinacia oleracea
5783
-
2.3.1.51
microsome
-
Spinacia oleracea
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.51
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.3.1.51
0.0051
-
-
Spinacia oleracea
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.51
-20░C, microsomal preparation, at least 3 weeks
Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
487188
Spinacia oleracea
?
2.3.1.51
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
?
2.3.1.51
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
?
2.3.1.51
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
CoA + ?
-
487188
Spinacia oleracea
?
2.3.1.51
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
CoA + ?
-
487188
Spinacia oleracea
?
2.3.1.51
additional information
the enzyme uses preferentially those acyl donors and acceptors, which contain oleate, specificity and selectivity experiments
487188
Spinacia oleracea
?
-
-
-
-
2.3.1.51
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
?
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
487188
Spinacia oleracea
?
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
2.3.1.51
25
-
assay at
Spinacia oleracea
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.51
9
11
broad, assay at pH 10
Spinacia oleracea