BRENDA - Enzyme Database

Facilitated utilization of endogenously synthesized lysophosphatidic acid by 1-acylglycerophosphate acyltransferase from Escherichia coli

Kessels, J.M.M.; Ousen, H.; Van den Bosch, H.; Biochim. Biophys. Acta 753, 227-235 (1983)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.51
membrane
-
Escherichia coli
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
Escherichia coli
involved in phospholipid biosynthesis
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
Escherichia coli
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.51
Escherichia coli
-
K12, strains: LCD45 and CE1061
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.51
partial, separated from EC 2.3.1.15 using Triton X-100, the enzyme is not solubilized by Triton X-100 or cholate
Escherichia coli
Renatured (Commentary)
EC Number
Commentary
Organism
2.3.1.51
by mixing 1 vol. of the fraction containing the enzyme with 4 vol. of sonicated phosphatidylcholine/phosphatidylglycerol or total E. coli lipid vesicles
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.3.1.51
0.0008
-
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
487180
Escherichia coli
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
involved in phospholipid biosynthesis
487180
Escherichia coli
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
?
2.3.1.51
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
?
2.3.1.51
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
CoA + ?
-
487180
Escherichia coli
?
2.3.1.51
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
CoA + ?
-
487180
Escherichia coli
?
2.3.1.51
additional information
lysophosphatidic acid, synthesized by EC 2.3.1.15, is utilized by the enzyme without prior mixing with the total membrane-associated pool of lysophosphatidic acid, and suggest a close proximity of the two enzymes in native E. coli membranes. This property of the acyltransferases is lost upon separation and reconstitution of enzyme activities
487180
Escherichia coli
?
-
-
-
-
2.3.1.51
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
?
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
?
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
2.3.1.51
30
-
assay at
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.51
9
-
assay at
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.51
membrane
-
Escherichia coli
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
Escherichia coli
involved in phospholipid biosynthesis
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
Escherichia coli
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.51
partial, separated from EC 2.3.1.15 using Triton X-100, the enzyme is not solubilized by Triton X-100 or cholate
Escherichia coli
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.51
by mixing 1 vol. of the fraction containing the enzyme with 4 vol. of sonicated phosphatidylcholine/phosphatidylglycerol or total E. coli lipid vesicles
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.3.1.51
0.0008
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
487180
Escherichia coli
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
involved in phospholipid biosynthesis
487180
Escherichia coli
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
?
2.3.1.51
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
?
2.3.1.51
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
CoA + ?
-
487180
Escherichia coli
?
2.3.1.51
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
CoA + ?
-
487180
Escherichia coli
?
2.3.1.51
additional information
lysophosphatidic acid, synthesized by EC 2.3.1.15, is utilized by the enzyme without prior mixing with the total membrane-associated pool of lysophosphatidic acid, and suggest a close proximity of the two enzymes in native E. coli membranes. This property of the acyltransferases is lost upon separation and reconstitution of enzyme activities
487180
Escherichia coli
?
-
-
-
-
2.3.1.51
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
?
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
487180
Escherichia coli
?
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
2.3.1.51
30
-
assay at
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.51
9
-
assay at
Escherichia coli