BRENDA - Enzyme Database

A direct nonchromatographic assay for 1-acyl-sn-glycerol-3-phosphate acyltransferase

Rajasekharan, R.; Ray, T.K.; Cronan, J.E.; Anal. Biochem. 173, 376-382 (1988)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.51
membrane
-
Escherichia coli
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
Escherichia coli
involved in phospholipid biosynthesis
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
Escherichia coli
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.51
Escherichia coli
-
K12, strains: LCD45 and CE1061
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.51
-
Escherichia coli
Renatured (Commentary)
EC Number
Commentary
Organism
2.3.1.51
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
i.e. lysophosphatidic acid, highly specific for 1-acyl-sn-glycerol 3-phosphate, catalyzes acylation of position 2 hydroxyl-group
487179
Escherichia coli
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
487179
Escherichia coli
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
involved in phospholipid biosynthesis
487179
Escherichia coli
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
487179
Escherichia coli
?
2.3.1.51
additional information
description of a direct nonchromatographic assay for the enzyme
487179
Escherichia coli
?
-
-
-
-
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487179
Escherichia coli
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
487179
Escherichia coli
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.51
30
-
assay at
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.51
9
-
assay at
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.51
membrane
-
Escherichia coli
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
Escherichia coli
involved in phospholipid biosynthesis
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
Escherichia coli
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.51
-
Escherichia coli
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.51
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
i.e. lysophosphatidic acid, highly specific for 1-acyl-sn-glycerol 3-phosphate, catalyzes acylation of position 2 hydroxyl-group
487179
Escherichia coli
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
i.e. phosphatidic acid
487179
Escherichia coli
?
2.3.1.51
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
involved in phospholipid biosynthesis
487179
Escherichia coli
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
487179
Escherichia coli
?
2.3.1.51
additional information
description of a direct nonchromatographic assay for the enzyme
487179
Escherichia coli
?
-
-
-
-
2.3.1.51
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
487179
Escherichia coli
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
487179
Escherichia coli
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.51
30
-
assay at
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.51
9
-
assay at
Escherichia coli