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Literature summary extracted from

  • Weiss, B.; Stoffel, W.
    Human and murine serine-palmitoyl-CoA transferase. Cloning, expression and characterization of the key enzyme in sphingolipid synthesis (1997), Eur. J. Biochem., 249, 239-247.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.50
-
 Homo sapiens
2.3.1.50 expression of mLCB1 and LCB2 as glutathione-S-transferase fusion protein in Escherichia coli DH5alpha Mus musculus
2.3.1.50 hLCB1 and hLCB2 Homo sapiens
2.3.1.50 in vitro transcription and translation of mLCB2 Mus musculus
2.3.1.50 overexpression of mLCB1 and mLCB2 in human embryonic kidney cells, independently and in coexpression with mLCB1 Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.50 beta-chloro-L-alanine
-
 Homo sapiens
2.3.1.50 beta-chloro-L-alanine
-
 Mus musculus
2.3.1.50 L-Cycloserine
-
 Homo sapiens
2.3.1.50 L-Cycloserine
-
 Mus musculus
2.3.1.50 lipoxamycin
-
 Homo sapiens
2.3.1.50 lipoxamycin
-
 Mus musculus
2.3.1.50 myriocin
-
 Homo sapiens
2.3.1.50 myriocin
-
 Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.50 palmitoyl-CoA + L-serine Mus musculus first step of biosynthesis of sphingolipid bases CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
2.3.1.50 palmitoyl-CoA + L-serine Homo sapiens first step of biosynthesis of sphingolipid bases CoA + 3-dehydro-D-sphinganine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.50 Homo sapiens
-
-
-
2.3.1.50 Homo sapiens
-
 gene hLCB1 and hLCB2
-
2.3.1.50 Mus musculus
-
 gene mLCB2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.50 no glycoprotein
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.50 kidney
-
 Mus musculus
-
2.3.1.50 kidney human embryonic kidney cell line HEK 293 Homo sapiens
-
2.3.1.50 lung
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.50 0.000018
-
 HEK cell extract Homo sapiens
2.3.1.50 0.000044
-
 HEK cells transfected with mLCB2, crude extract Mus musculus
2.3.1.50 0.000044
-
 HEK cells transfected with mLCB2, crude extract Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.50 palmitoyl-CoA + L-serine
-
 Mus musculus CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
2.3.1.50 palmitoyl-CoA + L-serine
-
 Homo sapiens CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
2.3.1.50 palmitoyl-CoA + L-serine first step of biosynthesis of sphingolipid bases Mus musculus CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
2.3.1.50 palmitoyl-CoA + L-serine first step of biosynthesis of sphingolipid bases Homo sapiens CoA + 3-dehydro-D-sphinganine + CO2
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.50 pyridoxal 5'-phosphate
-
 Mus musculus
2.3.1.50 pyridoxal 5'-phosphate
-
 Homo sapiens