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Literature summary extracted from

  • Andres, H.H.; Kolb, H.J.; Weiss, L.
    Purification and physical-chemical properties of acetyl-CoA:arylamine N-acetyltransferase from pigeon liver (1983), Biochim. Biophys. Acta, 746, 182-192.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.5 dithiothreitol stabilizes Columba sp.
2.3.1.5 EDTA stabilizes Columba sp.
2.3.1.5 repeated freeze-thawing inactivates Columba sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.5 DTNB
-
 Columba sp.
2.3.1.5 methopterin kinetics Columba sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.5 additional information
-
 amino acid analysis Columba sp.
2.3.1.5 31900
-
 1 * 31900, SDS-PAGE Columba sp.
2.3.1.5 32900
-
 high-speed sedimentation equilibrium analysis Columba sp.
2.3.1.5 33000
-
 gel filtration Columba sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.5 acetyl-CoA + an arylamine Columba sp. constitutive enzyme CoA + a N-acetylarylamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.5 Columba sp.
-
 pigeon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.5 affinity chromatography Columba sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.5 liver
-
 Columba sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.5 14.02
-
-
 Columba sp.

Storage Stability

EC Number Storage Stability Organism
2.3.1.5 -70°C, frozen at neutral pH in liquid nitrogen, no loss of activity over a long-time range Columba sp.
2.3.1.5 4°C, several days in various buffer systems at pH 6.0-8.5 Columba sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.5 acetyl-CoA + 4-nitroaniline
-
 Columba sp. CoA + N-acetyl-4-nitroaniline
-
 ?
2.3.1.5 acetyl-CoA + an arylamine constitutive enzyme Columba sp. CoA + a N-acetylarylamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.5 monomer 1 * 31900, SDS-PAGE Columba sp.
2.3.1.5 More enzyme forms dimer under conditions of catalysis Columba sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.5 additional information
-
 pI: 4.8 Columba sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.5 6 8.5 in several buffer systems, at 4°C, several days stable Columba sp.